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Spectroscopy and Spectral Analysis, Volume. 42, Issue 10, 3031(2022)

Wavelength Selection Method of Algal Fluorescence Spectrum Based on Convex Point Extraction From Feature Region

Yong-bin ZHANG1、*, Dan-dan ZHU1、1;, Ying CHEN1、1; *;, Zhe LIU1、1;, Wei-liang DUAN1、1;, and Shao-hua LI2、2;
Author Affiliations
  • 11. Hebei Province Key Laboratory of Test/Measurement Technology and Instrument, School of Electrical Engineering, Yanshan University, Qinhuangdao 066004, China
  • 22. Hebei Sailhero Environmental Protection Hi-tech Co., Ltd., Shijiazhuang 050000, China
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    Figures & Tables(12)
    Contour spectra of 3 phytoplankton(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 1. Contour spectra of 3 phytoplankton
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    Excitation spectra of 3 phytoplankton before smooth denoising(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 2. Excitation spectra of 3 phytoplankton before smooth denoising
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    Excitation spectra of 3 phytoplankton after smooth denoising(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 3. Excitation spectra of 3 phytoplankton after smooth denoising
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    Mahalanobis distance distribution of 3 phytoplankton(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 4. Mahalanobis distance distribution of 3 phytoplankton
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    Concentration residual distribution of 3 phytoplankton(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 5. Concentration residual distribution of 3 phytoplankton
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    Convex pointsstatistics of 3 phytoplankton(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 6. Convex pointsstatistics of 3 phytoplankton
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    Initial candidate feature region(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 7. Initial candidate feature region
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    Statistics of convex points under the initial candidate feature region(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 8. Statistics of convex points under the initial candidate feature region
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    Change curve of RMSECV with different ηq and ηt(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 9. Change curve of RMSECV with different ηq and ηt
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    Wavelength selection results of feature region combined with convex point extraction(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 10. Wavelength selection results of feature region combined with convex point extraction
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    Wavelength selection results of UVE(a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

    Fig. 11. Wavelength selection results of UVE
    (a): Aureococcus anophagefferens; (b): Chlorella vulgaris; (c): Synechococcus elongatus

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    • Table 1. R2 and RMSECV of three methods

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      Table 1. R2 and RMSECV of three methods

      PhytoplanktonMethodWavelength
      point      		R2RMSECV/
      (106 cells·mL-1)
      Aureococcus
      anophagefferens      		Total-PLS1 0710.81431.55
      Feature region+convex point-PLS770.830 71.37
      UVE-PLS6760.816 21.53
      Chlorella
      vulgaris      		Total-PLS1 0710.983 32.80
      Feature region+convex point-PLS750.985 32.60
      UVE-PLS4320.984 92.67
      Synechococcus
      elongatus      		Total-PLS1 0710.875 21.76
      Feature region+convex point-PLS670.907 61.50
      UVE-PLS3840.895 31.66
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    Yong-bin ZHANG, Dan-dan ZHU, Ying CHEN, Zhe LIU, Wei-liang DUAN, Shao-hua LI. Wavelength Selection Method of Algal Fluorescence Spectrum Based on Convex Point Extraction From Feature Region[J]. Spectroscopy and Spectral Analysis, 2022, 42(10): 3031

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    Paper Information

    Category: Research Articles

    Received: Aug. 9, 2021

    Accepted: Oct. 22, 2021

    Published Online: Nov. 23, 2022

    The Author Email: Yong-bin ZHANG (2824272678@qq.com)

    DOI:10.3964/j.issn.1000-0593(2022)10-3031-08

    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology