Acta Laser Biology Sinica, Volume. 33, Issue 5, 400(2024)
Synthetic Biology Applications for Enhancing the Efficient Production of Spinosad in Saccharopolyspora spinosa
[1] [1] SJOBLAD R D, MCCLINTOCK J T, ENGLER R. Toxicological considerations for protein components of biological pesticide products[J]. Regulatory Toxicology and Pharmacology, 1992, 15(1): 3-9.
[2] [2] BASS C, JONES C M. Editorial overview: pests and resistance: resistance to pesticides in arthropod crop pests and disease vectors: mechanisms, models and tools[J]. Current Opinion in Insect Science, 2018, 27: 4-7.
[3] [3] MOSSA A H, MOHAFRASH S M M, CHANDRASEKARAN N. Safety of natural insecticides: toxic effects on experimental animals[J]. Biomed Research International, 2018, 2018: 54-70.
[4] [4] SALWAN R, SHARMA V. Molecular and biotechnological aspects of secondary metabolites in actinobacteria[J]. Microbiological Research, 2020, 231: 74-91.
[5] [5] MERTZ F P, YAO R C. Saccharopolyspora spinosa sp. nov. isolated from soil collected in a sugar mill rum still[J]. International Journal of Systematic and Evolutionary Microbiology, 1990, 40(1): 34-39.
[6] [6] LEWER P, HAHN D R, KARR L L, et al. Discovery of the butenyl-spinosyn insecticides: novel macrolides from the new bacterial strain Saccharopolyspora pogona[J]. Bioorganic & Medicinal Chemistry, 2009, 17(12): 4185-4196.
[7] [7] MILLAR N S, DENHOLM I. Nicotinic acetylcholine receptors: targets for commercially important insecticides[J]. Invertebrate Neuroscience, 2007, 7(1): 53-66.
[8] [8] DEAMICIS C V, DRIPPS J E, HATTON C J, et al. Physical and biological properties of the spinosyns: novel macrolide pest-control agents from fermentation[J]. ACS Symposium Series, 1997,658: 144-154.
[9] [9] HUANG K X, XIA L, ZHANG Y, et al. Recent advances in the biochemistry of spinosyns[J]. Applied Microbiology and Biotechnology, 2009, 82(1): 13-23.
[10] [10] WANG L, ZANG X, ZHOU J. Synthetic biology: a powerful booster for future agriculture[J]. Advanced Agrochem, 2022, 1(1): 7-11.
[11] [11] ZABALA D, BRANA A F, SALAS J A, et al. Increasing antibiotic production yields by favoring the biosynthesis of precursor metabolites glucose-1-phosphate and/or malonyl-CoA in Streptomyces producer strains[J]. The Journal of Antibiotics, 2016, 69(3): 179-182.
[12] [12] ZHAO C, HUANG Y, GUO C, et al. Heterologous expression of spinosyn biosynthetic gene cluster in Streptomyces species is dependent on the expression of rhamnose biosynthesis genes[J]. Journal of Molecular Microbiology and Biotechnology, 2017, 27(3): 190-198.
[13] [13] TAN G Y, DENG K, LIU X, et al. Heterologous biosynthesis of spinosad: an omics-guided large polyketide synthase gene cluster reconstitution in Streptomyces[J]. ACS Synthetic Biology, 2017,6(6): 995-1005.
[14] [14] SONG C, LUAN J, CUI Q, et al. Enhanced heterologous spinosad production from a 79-kb synthetic multioperon assembly[J]. ACS Synthetic Biology, 2019, 8(1): 137-147.
[15] [15] LI X, GUO R, LUAN J, et al. Improving spinosad production by tuning expressions of the forosamine methyltransferase and the forosaminyl transferase to reduce undesired less active byproducts in the heterologous host Streptomyces albus J1074[J]. Microbial Cell Factories, 2023, 22(1): 15-21.
[16] [16] AN Z, TAO H, WANG Y, et al. Increasing the heterologous production of spinosad in Streptomyces albus J1074 by regulating biosynthesis of its polyketide skeleton[J]. Synthetic and Systems Biotechnology, 2021, 6(4): 292-301.
[17] [17] LI H, PAN Y, LIU G. Multiplying the heterologous production of spinosad through tandem amplification of its biosynthetic gene cluster in Streptomyces coelicolor[J]. Microbial Biotechnology, 2022, 15(5): 1550-1560.
[19] [19] HUANG J, YU Z, LI M H, et al. High level of spinosad production in the heterologous host Saccharopolyspora erythraea[J]. Applied and Environmental Microbiology, 2016, 82(18): 5603-5611.
[20] [20] WANG X, ZHANG C, WANG M, et al. Genome-scale metabolic network reconstruction of Saccharopolyspora spinosa for spinosad production improvement[J]. Microbial Cell Factories, 2014, 13(1): 41-47.
[21] [21] LIU Z, ZHU Z, TANG J, et al. RNA-seq-based transcriptomic analysis of Saccharopolyspora spinosa revealed the critical function of PEP phosphonomutase in the replenishment pathway[J]. Journal of Agricultural and Food Chemistry, 2020, 68(49): 14660-14669.
[22] [22] ZHANG Y, LIU X, YIN T, et al. Comparative transcriptomic analysis of two Saccharopolyspora spinosa strains reveals the relationships between primary metabolism and spinosad production[J]. Scientific Reports, 2021, 11(1): 14779-14792.
[23] [23] LUO Y, DING X, XIA L, et al. Comparative proteomic analysis of Saccharopolyspora spinosa SP06081 and PR2 strains reveals the differentially expressed proteins correlated with the increase of spinosad yield[J]. Proteome Science, 2011, 9(1): 40-51.
[24] [24] ZHAO F, XUE C, WANG M, et al. A comparative metabolomics analysis of Saccharopolyspora spinosa WT, WH124, and LU104 revealed metabolic mechanisms correlated with increases in spinosad yield[J]. Bioscience Biotechnology and Biochemistry, 2013, 77(8): 1661-1668.
[25] [25] RANG J, CAO L, SHUAI L, et al. Promoting butenyl-spinosyn production based on omics research and metabolic network construction in Saccharopolyspora pogona[J]. Journal of Agricultural and Food Chemistry, 2022, 70(11): 3557-3567.
[26] [26] HE H, TANG J, CHEN J, et al. Flaviolin-like gene cluster deletion optimized the butenyl-spinosyn biosynthesis route in Saccharopolyspora pogona[J]. ACS Synthetic Biology, 2021, 10(10): 2740-2752.
[27] [27] TANG J, HE H, LI Y, et al. Comparative proteomics reveals the effect of the transcriptional regulator Sp13016 on butenyl-spinosyn biosynthesis in Saccharopolyspora pogona[J]. Journal of Agricultural and Food Chemistry, 2021, 69(42): 12554-12565.
[28] [28] MADDURI K, WALDRON C, MERLO D J. Rhamnose biosynthesis pathway supplies precursors for primary and secondary metabolism in Saccharopolyspora spinosa[J]. Journal of Bacteriology, 2001, 183(19): 5632-5638.
[29] [29] XUE C, DUAN Y, ZHAO F, et al. Stepwise increase of spinosad production in Saccharopolyspora spinosa by metabolic engineering[J]. Biochemical Engineering Journal, 2013, 72: 90-95.
[30] [30] TANG Y, XIA L, DING X, et al. Duplication of partial spinosyn biosynthetic gene cluster in Saccharopolyspora spinosa enhances spinosyn production[J]. FEMS Microbiology Letters, 2011,325(1): 22-29.
[31] [31] BRIDGET A F, NGUYEN C T, MAGAR R T, et al. Increasing production of spinosad in Saccharopolyspora spinosa by metabolic engineering[J]. Biotechnology and Applied Biochemistry, 2023, 70(3): 1035-1043.
[32] [32] PAN H X, LI J A, HE N J, et al. Improvement of spinosad production by overexpression of gtt and gdh controlled by promoter PermE* in Saccharopolyspora spinosa SIPI-A2090[J]. Biotechnology Letters, 2011, 33(4): 733-739.
[33] [33] JHA A K, POKHREL A R, CHAUDHARY A K, et al. Metabolic engineering of rational screened Saccharopolyspora spinosa for the enhancement of spinosyns A and D production[J]. Molecules and Cells, 2014, 37(10): 727-733.
[34] [34] LIU G, CHATER K F, CHANDRA G, et al. Molecular regulation of antibiotic biosynthesis in Streptomyces[J]. Microbiology and Molecular Biology Reviews, 2013, 77(1): 112-143.
[35] [35] WANG W, LI S, LI Z, et al. Harnessing the intracellular triacylglycerols for titer improvement of polyketides in Streptomyces[J]. Nature Biotechnology, 2020, 38(1): 76-83.
[36] [36] XUE C, ZHANG X, YU Z, et al. Up-regulated spinosad pathway coupling with the increased concentration of acetyl-CoA and malonyl-CoA contributed to the increase of spinosad in the presence of exogenous fatty acid[J]. Biochemical Engineering Journal, 2013,81: 47-53.
[37] [37] GUOJUN Y, YUPING H, YAN J, et al. A new medium for improving spinosad production by Saccharopolyspora spinosa[J]. Jundishapur Journal of Microbiology, 2016, 9(6): e16765-e16769.
[40] [40] MCLEAN K J, HANS M, MEIJRINK B, et al. Single-step fermentative production of the cholesterol-lowering drug pravastatin via reprogramming of Penicillium chrysogenum[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(9): 2847-2852.
[41] [41] WAN M Y, PENG C, DING W X, et al. Calcium-phosphate combination enhances spinosad production in Saccharopolyspora spinosa via regulation of fatty acid metabolism[J]. Applied Biochemistry and Biotechnology, 2022, 194(6): 2528-2541.
[42] [42] WHITE D, ELLIOTT S, ODEAN E, et al. Mycobacterium tuberculosis Pst/SenX3-RegX3 regulates membrane vesicle production independently of ESX-5 activity[J]. mBio, 2018, 9(3): 778-791.
[43] [43] AGGARWAL S, SOMANI V K, GUPTA V, et al. Functional characterization of PhoPR two component system and its implication in regulating phosphate homeostasis in Bacillus anthracis[J]. BBA - General Subjects, 2017, 1861(1): 2956-2970.
[44] [44] RANG J, HE H, CHEN J, et al. SenX3-RegX3, an important two-component system, regulates strain growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona[J]. iScience, 2020,23(8): 101398-101443.
[45] [45] TANG J, CHEN J, LIU Y, et al. The global regulator PhoU positively controls growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona[J]. Frontiers in Microbiology, 2022,13: 904627-904637.
[46] [46] YANG Q, DING X, LIU X, et al. Differential proteomic profiling reveals regulatory proteins and novel links between primary metabolism and spinosad production in Saccharopolyspora spinosa[J]. Microbial Cell Factories, 2014, 13(1): 27-42.
[48] [48] RANG J, XIA Z, SHUAI L, et al. A TetR family transcriptional regulator, SP_2854 can affect the butenyl-spinosyn biosynthesis by regulating glucose metabolism in Saccharopolyspora pogona[J]. Microbial Cell Factories, 2022, 21(1): 83-99.
[49] [49] HE H, YUAN S, HU J, et al. Effect of the TetR family transcriptional regulator Sp1418 on the global metabolic network of Saccharopolyspora pogona[J]. Microbial Cell Factories, 2020, 19(1): 27-37.
[50] [50] LIU Z, XIAO J, TANG J, et al. Effects of acuC on the growth development and spinosad biosynthesis of Saccharopolyspora spinosa[J]. Microbial Cell Factories, 2021, 20(1): 141-155.
[52] [52] CAO L, ZHU Z, QIN H, et al. Effects of a Pirin-like protein on strain growth and spinosad biosynthesis in Saccharopolyspora spinosa[J]. Applied Microbiology and Biotechnology, 2023,107(17): 5439-5451.
[53] [53] HU J, XIA Z, SHUAI L, et al. Effect of pII key nitrogen regulatory gene on strain growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona[J]. Applied Microbiology and Biotechnology, 2022, 106(8): 3081-3091.
[54] [54] LI L, RANG J, HE H, et al. Impact on strain growth and butenylspinosyn biosynthesis by overexpression of polynucleotide phosphorylase gene in Saccharopolyspora pogona[J]. Applied Microbiology and Biotechnology, 2018, 102(18): 8011-8021.
[57] [57] YANG Q, LI Y, YANG H, et al. Proteomic insights into metabolic adaptation to deletion of metE in Saccharopolyspora spinosa[J]. Applied Microbiology and Biotechnology, 2015, 99(20): 8629-8641.
[59] [59] TANG J, ZHU Z, HE H, et al. Bacterioferritin: a key iron storage modulator that affects strain growth and butenyl-spinosyn biosynthesis in Saccharopolyspora pogona[J]. Microbial Cell Factories, 2021, 20(1): 157-173.
[61] [61] RANG J, ZHU Z, LI Y, et al. Identification of a TetR family regulator and a polyketide synthase gene cluster involved in growth development and butenyl-spinosyn biosynthesis of Saccharopolyspora pogona[J]. Applied Microbiology and Biotechnology, 2021, 105(4): 1519-1533.
[62] [62] RANG J, LI Y, CAO L, et al. Deletion of a hybrid NRPS-T1PKS biosynthetic gene cluster via Latour gene knockout system in Saccharopolyspora pogona and its effect on butenyl-spinosyn biosynthesis and growth development[J]. Microbial Biotechnology, 2021, 14(6): 2369-2384.
[63] [63] YANG R, LIU X, WEN Y, et al. The PhoP transcription factor negatively regulates avermectin biosynthesis in Streptomyces avermitilis[J]. Applied Microbiology and Biotechnology, 2015,99(24): 10547-10557.
[64] [64] DANG F, XU Q, QIN Z, et al. Rationally improving doramectin production in industrial Streptomyces avermitilis strains[J]. Bioengineering (Basel), 2023, 10(6): 739-749.
Get Citation
Copy Citation Text
FANG Jing, RANG Jie, XIA Liqiu. Synthetic Biology Applications for Enhancing the Efficient Production of Spinosad in Saccharopolyspora spinosa[J]. Acta Laser Biology Sinica, 2024, 33(5): 400
Category:
Received: Apr. 12, 2024
Accepted: Dec. 10, 2024
Published Online: Dec. 10, 2024
The Author Email: Liqiu XIA (xialq@hunnu.edu.cn)