Acta Photonica Sinica, Volume. 54, Issue 8, 0830001(2025)
Research on the Construction and Method of Fluorescence In-situ Selective Quantitative Detection System for Pesticide Pollutants in Groundwater
Figures & Tables(21)
Fig. 1. The three-dimensional fluorescence spectra of five pesticides
Fig. 2. Comparison of correlation and detection limits between concentrations and signals of five pesticides
Fig. 8. Synchronous integrated fluorescence signal detection circuit
Fig. 10. Detection sensitivity and linear correlation of the integrated circuit
Fig. 11. Relationship between integration time and output in an integrating circuit
Fig. 12. Plots of fluorescence intensity versus concentration of five pesticides
Fig. 13. Plots of fluorescence intensity versus concentration of five pesticides by Hitachi F-7000 fluorescence spectrometer
Table 1. The main reagents
View tableView in ArticleTable 1. The main reagents
Chemical agent Molecular formula Purity Production company Chorothalonil C8Cl4N2 ≥99% Shanghai Aladdin Biochemical Technology Co.， Ltd Chlorpyrifos C9H11Cl3NO3PS ≥99% Shanghai Aladdin Biochemical Technology Co.， Ltd Fuberidazole C11H8N2O ≥99% Shanghai Aladdin Biochemical Technology Co.， Ltd Carbaryl C12H11NO2 ≥99% Shanghai Aladdin Biochemical Technology Co.， Ltd Carbendazim C9H9N3O2 ≥99% Shanghai Aladdin Biochemical Technology Co.， Ltd
Table 2. Linear regression equation， fitting coefficient， detection limit of five pesticides for each channel
View tableView in ArticleTable 2. Linear regression equation， fitting coefficient， detection limit of five pesticides for each channel
Pesticides Light path channel Linear Fitting factor/R2 LOD/（μg·L-1 ） Chorothalonil 255~340
265~340
y=74.04x-257.15
y=37.29x-286.96
0.997 9
0.997 3
0.1
0.3
Chlorpyrifos 265~340
285~340
y=1.12x+36.12
y=7.28x+181.06
0.998 4
0.990 3
6.5
6.5
Fuberidazole 255~340
265~340
285~340
y=5 495.78x-792.33
y=4 828.34x-703.10
y=19 435.60x-3 210.1
0.999 5
0.999 5
0.999 9
0.002
0.003
0.002
Carbaryl 255~340
265~340
285~340
y=197.82x-151.90
y=291.24x-183.87
y=625.13x-742.44
0.999 8
0.999 9
0.999 8
0.05
0.04
0.08
Carbendazim 255~340
265~340
285~340
y=2.43x-88.65
y=3.48x+493.64
y=9.48x+80.92
0.996 8
0.996 8
0.997 4
2.01
1.7
1.7
Table 3. Linear regression equation， fitting coefficient， detection limit of five pesticides by Hitachi F-7000 fluorescence spectrometer
View tableView in ArticleTable 3. Linear regression equation， fitting coefficient， detection limit of five pesticides by Hitachi F-7000 fluorescence spectrometer
Pesticides Light path channel Linear Fitting factor/R2 LOD/（μg·L-1 ） Chorothalonil 255~340
265~340
y=2.77x+22.02
y=1.85x+15.81
0.994 4
0.996 4
2.6
3.2
Chlorpyrifos 285~340 y=0.22x+8.44 0.974 2 26.0 Fuberidazole 255~340
265~340
285~340
y=246.16x-39.27
y=223.09x-21.64
y=462.28x-43.49
0.991 3
0.995 5
0.995 0
0.03
0.03
0.02
Carbaryl 255~340
265~340
285~340
y=16.91x-62.64
y=29.76x+16.09
y=35.91x-20.66
0.975 2
0.995 3
0.996 6
0.4
0.2
0.2
Carbendazim 255~340
265~340
285~340
y=0.24x+19.54
y=0.32x+21.76
y=0.73x+59.52
0.978 6
0.991 4
0.982 6
30.3
18.6
7.8
Table 4. Linear regression equation， fitting coefficient， detection limit for target pesticides
View tableView in ArticleTable 4. Linear regression equation， fitting coefficient， detection limit for target pesticides
Pesticides Ligh tpath channel Linear Fitting factor/R2 LOD/（μg·L-1 ） Chorothalonil 255~340
265~340
y=46.62x-8.06
y=29.12x-155.15
0.997 3
0.998 1
1.3
0.4
Chlorpyrifos 265~340
285~340
y=0.93x+106.84
y=7x+6.42
0.955 7
0.998 2
12.7
6.9
Fuberidazole 255~340
265~340
285~340
y=4 085.11x-154.49
y=3 942.02x-109.53
y=15 124.92x-314.75
0.999 9
0.998 9
0.999 8
0.01
0.003
0.002
Carbaryl 255~340
265~340
285~340
y=108.43x+1 694.19
y=202.86x-915.74
y=198.55x+2 523.79
0.984 0
0.976 6
0.976 5
0.6
0.06
0.1
Carbendazim
255~340
265~340
285~340
y=3.77x+92.72
y=5.56x+19.41
y=14.24x+730.74
0.995 1
0.958 9
0.990 8
6.6
2.1
1.9
Table 5. Results of qualitative model analysis
View tableView in ArticleTable 5. Results of qualitative model analysis
Component Test Set_totol Test Set_real Accuracy rate One-component sample 40 37 92.5% Mixed samples of Chlorothalonil， Chlorpyrifos， Fuberidazole，
Carbaryl and Carbendazim
28 27 96.4% Chlorpyrifos mixed with Fuberidazole， Carbarydine and
Carbendazim
20 18 90% Mixed samples of Fuberidazole， Carbaryl and Carbendazim 12 11 91.7% Carbaryl mixed with Carbendazim 8 8 100%
Table 6. Results of quantitative model analysis
View tableView in ArticleTable 6. Results of quantitative model analysis
Component MAE M10%~20% M0%~10% One-component sample 0.8% 3/40 37/40 Mixed samples of Chlorothalonil， Chlorpyrifos， Fuberidazole， Carbaryl and Carbendazim 2.4% 0/28 25/28 Chlorpyrifos mixed with Fuberidazole，
Carbarydine and Carbendazim
4.9% 6/20 14/20 Mixed samples of Fuberidazole， Carbaryl and Carbendazim 15.6% 4/12 8/12 Carbaryl mixed with carbendazim 10.6% 6/8 2/8
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Hengxin SONG, Ruifang YANG, Yuxi JIANG, Nanjing ZHAO, Gaofang YIN, Jingbo DUAN, Ming GAO, Yingchong WANG. Research on the Construction and Method of Fluorescence In-situ Selective Quantitative Detection System for Pesticide Pollutants in Groundwater[J]. Acta Photonica Sinica, 2025, 54(8): 0830001
Paper Information
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Received: Feb. 24, 2025
Accepted: Apr. 29, 2025
Published Online: Sep. 26, 2025
The Author Email: Ruifang YANG (rfyang@aiofm.ac.cn), Nanjing ZHAO (njzhao@aiofm.ac.cn)
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