Diagnosis of Breast Cancer Using Gaussian Function to Fit Autofluorescence Spectrum

Wenjing Chen; Zhaohang Jiao; Dongli Qi; Longhai Shen; Nuo Xu; Dongfei Xie; Yifei Li; Jiahua You; Qi Li; Yu Feng

doi:10.3788/CJL202249.2007106

Chinese Journal of Lasers, Volume. 49, Issue 20, 2007106(2022)

Diagnosis of Breast Cancer Using Gaussian Function to Fit Autofluorescence Spectrum

Wenjing Chen, Zhaohang Jiao, Dongli Qi, Longhai Shen, Nuo Xu, Dongfei Xie, Yifei Li, Jiahua You, Qi Li, and Yu Feng^*

Author Affiliations

College of Science, Shenyang Ligong University, Shenyang 110158, Liaoning, China

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Abstract Get PDF(in Chinese)

Figures & Tables(7)

Fig. 1. Schematic of experimental device

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Fig. 2. Fluorescence spectra of slide and stain. (a) Fluorescence spectra of slide; (b) fluorescence spectra of stain

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Fig. 3. Normalized mean fluorescence spectra of breast tissues

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Fig. 4. Normalized mean fluorescence spectrograms fitted by Gaussian function. (a) Normal breast tissue by SP staining; (b) unstained cancerous breast tissue; (c) cancerous breast tissue by HE staining; (d) cancerous breast tissue by SP staining

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Table 1. Experimental instrument information table

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Table 1. Experimental instrument information table

Name	Instrument information
Laser	Wavelength: 405 nm; power: 150 mW
Dichroic mirror	Critical wavelength: 410 nm; transmission wavelength: >419 nm; transmittance: >80%; reflection wavelength: 360－405 nm; reflectance: >99%
Fiber probe	Diameter: 400 m; wavelength range: 300－800 nm
Fiber spectrometer	Spectral range: 200－1100 nm; scanning speed: 4500 scan·s^－1: resolution: 0.8 nm

Table 2. Characteristic parameter information of Gaussian fitting peak

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Table 2. Characteristic parameter information of Gaussian fitting peak

Sample	A₁	A₂	A₃	λ₁	λ₂	λ₃	ω₁	ω₂	ω₃	R²
SP(normal)	55.73	37.59	10.61	491.39	516.76	631.68	64.06	107.37	89.16	0.998
Unstained(cancer)	19.48	72.23	31.49	492.74	520.77	630.59	39.41	82.59	147.65	0.987
HE(cancer)	22.94	70.61	23.81	492.35	520.89	631.24	41.38	135.55	147.13	0.992
SP(cancer)	24.47	38.57	27.86	495.14	517.25	640.84	64.34	45.05	116.20	0.996

Table 3. Peak area ratios of A₅₁₇/A₄₉₂ and A₆₃₅/A₄₉₂ of normal and cancerous breast tissues

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Table 3. Peak area ratios of A₅₁₇/A₄₉₂ and A₆₃₅/A₄₉₂ of normal and cancerous breast tissues

Breast tissue	A₅₁₇/A₄₉₂	A₆₃₅/A₄₉₂
Normal tissue with SP staining	0.6745	0.1904
Unstained cancerous tissue	3.7079	1.6105
Cancerous tissue with HE staining	3.0780	1.0379
Cancerous tissue with SP staining	1.5762	1.1385

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Wenjing Chen, Zhaohang Jiao, Dongli Qi, Longhai Shen, Nuo Xu, Dongfei Xie, Yifei Li, Jiahua You, Qi Li, Yu Feng. Diagnosis of Breast Cancer Using Gaussian Function to Fit Autofluorescence Spectrum[J]. Chinese Journal of Lasers, 2022, 49(20): 2007106

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Paper Information

Category: Optical Diagnostics and Therapy

Received: May. 16, 2022

Accepted: Jul. 20, 2022

Published Online: Sep. 20, 2022

The Author Email: Feng Yu (14025921@qq.com)

DOI:10.3788/CJL202249.2007106

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Experimental instrument information table

Table 1. Experimental instrument information table

Table 2. Characteristic parameter information of Gaussian fitting peak

Table 2. Characteristic parameter information of Gaussian fitting peak

Table 3. Peak area ratios of A517/A492 and A635/A492 of normal and cancerous breast tissues

Table 3. Peak area ratios of A517/A492 and A635/A492 of normal and cancerous breast tissues

Table 3. Peak area ratios of A₅₁₇/A₄₉₂ and A₆₃₅/A₄₉₂ of normal and cancerous breast tissues

Table 3. Peak area ratios of A₅₁₇/A₄₉₂ and A₆₃₅/A₄₉₂ of normal and cancerous breast tissues