Spectroscopy and Spectral Analysis, Volume. 40, Issue 12, 3670(2020)
Study on Identification of Four Acute Leukemia Cell Lines Based on Laser Raman Spectroscopy
Figures & Tables(13)
Fig. 2. Two-dimentional and three-dimentional score scatter figure comparison of PCA and PLS-DA result of the four groups
(a): PCA two-dimentional score scatter figure; (b): PLS-DA two-dimentional score scatter figure;(c): PCA three-dimentional score scatter figure; (d): PLS-DA three-dimentional score scatter figure
Fig. 3. Comparison of PCA and PLS-DA result of the four groups
(a): PCA column figure; (b): PLS-DA column figure; (c): PCA loading line figure;(d): PLS-DA loading line figure; (e): PCA loading column figure; (f): PLS-DA loading column figure;(g): PCA cluster figure; (h): PLS-DA cluster figure
Fig. 4. Two-dimentional score figure for PCA model from two combinations of the four groups
Fig. 5. Three-dimentional score figure for PCA model from two combinations of the four groups
Fig. 6. Two-dimentional score figure for PLS-DA model from two combinations of the four groups
Fig. 7. Three-dimentional score figure for PLS-DA model from two combinations of the four groups
Fig. 8. Loading figure for PLS-DA model from two combinations of the four groups
Fig. 9. Dendrogram of cluster analysis from two combinations of the four groups using PCA
Fig. 10. Dendrogram of cluster analysis from two combinations of the four groups using PLS-DA
Table 1. Molecular structure and composition of Raman peaks
View tableView in ArticleTable 1. Molecular structure and composition of Raman peaks
Peak position
/cm-1Nucleic Acids Proteins Lipids Carbohydrates 622 phenylalanine 643 tyrosine 728 A C—S CH2 CH2 769 Tryptophan 789 O—P—O, C, U, T 826 O—P—O stretch 844 Glucose 852 tyrosine 920 proline ring/glucose/lactic acid 934 C—C 957 Hydroxyapatite/carotenoid/cholesterol 966~971 Hydroxyapatite 1 003 phenylalanine 1 031 Phenylalanine 1 048~1 050 C—H 1 060~1 195 C-C C-N C-C 1 087~1 101 C-C C-C 1 118 C-C 1 141 C-C 1 155 C-C(C-N) 1 158~1 171 Tyrosine C-C(C-N) 1 173 Tyrosine carbohydrates 1 206 Tryptophan 1 240~1 243 AmideⅢ 1 255~1 258 Amide Ⅲ 1 313 CH3 CH2 CH3 CH2 1 340 Polynucleotide 1 415 A, G 1 450 CH2 1 485 A, G 1 538~1539 C=C carotenoid 1 547~1 548 Tryptophan 1 581 Pyrimidine 1 658 AmideⅠ C=C
Table 2. Model quality parameter for PCA model from two combinations of the four groups
View tableView in ArticleTable 2. Model quality parameter for PCA model from two combinations of the four groups
Two combinations R2X Q2 Jurkat vs KG-1α 0.43 0.095 4 Jurkat vs NB4 0.514 0.093 6 Jurkat vs THP-1 0.467 0.149 KG-1α vs NB4 0.515 0.099 6 THP-1 vs KG-1α 0.519 0.163 THP-1 vs NB4 0.581 0.11
Table 3. Model quality parameter for PLS-DA model from two combinations of the four groups
View tableView in ArticleTable 3. Model quality parameter for PLS-DA model from two combinations of the four groups
Two combinations R2X R2Y Q2 Jurkat vs KG-1α 0.387 0.996 0.884 Jurkat vs NB4 0.31 0.963 0.817 Jurkat vs THP-1 0.199 0.91 0.788 KG-1α vs NB4 0.18 0.885 0.706 THP-1 vs KG-1α 0.472 0.993 0.94 THP-1 vs NB4 0.427 0.995 0.92
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Hao-yue LIANG, Xue-lian CHENG, Wan-zhu YANG, Wei WEN, Shu-xu DONG, Shi-xuan ZHAO, Yong-xin RU. Study on Identification of Four Acute Leukemia Cell Lines Based on Laser Raman Spectroscopy[J]. Spectroscopy and Spectral Analysis, 2020, 40(12): 3670
Paper Information
Category: Research Articles
Received: Nov. 3, 2019
Accepted: --
Published Online: Jun. 18, 2021
The Author Email: Hao-yue LIANG (lianghaoyue@ihcams.ac.cn)
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