In order to excavate and cultivate excellent strains of Stropharia rugosoannulata with high yield, high quality and suitable for cultivation in cold regions, four strains were introduced from Sichuan Province, Fujian Province, Henan Province and other places. Through mycelium culture, cultivation, field experiment and regional adaptability test, the agronomic traits of different strains were evaluated. The experimental results indicated that among the four introduced strains, DQ5 demonstrated rapid mycelial growth rate, shorter cultivation cycle, and strong resistance to contaminants. Under both interplant with Zea mays and greenhouse cultivation systems, DQ5 exhibited high and stable yields with superior fruit bodies morphology. In regional adaptability trials, the average yield of DQ5 under greenhouse cultivation reached 10.46 kg·m^-2, with first-grade mushrooms accounting for over 35% of total production. However, the local dominant strain DQ3 maintained better performance in open-field and forest-understory cultivation modes. Comprehensive analysis suggests that the introduced DQ5 represents an excellent strain suitable for facility-based cultivation in cold regions, demonstrating broad potential for application and extension across most areas of Heilongjiang Province.