Laser & Optoelectronics Progress, Volume. 62, Issue 2, 0237015(2025)

Large Field-of-View Light-Sheet Image Reconstruction Based on Model-Driven Deconvolutional Network

Haoyang Wu1、*, Xiaojun Zhao2, and Xiaoquan Yang2
Author Affiliations
  • 1School of Biomedical Engineering, Hainan University, Haikou 570200, Hainan , China
  • 2Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, Hubei , China
  • show less
    Figures & Tables(9)
    Workflow of a position-related deconvolution network for reconstructing large FOV images
    Large FOV images obtained by axial swept light-sheet microscopy. (a)(b) Images of fluorescent microspheres; (c)(d) images of mouse brain slices
    PSF used in this experiment and normalized intensity profiles along the dashed line
    Network training error curves
    Examples of artifact problem in DWDN on simulated datasets
    Comparison of X-resolution between raw and reconstruction images with a large FOV on the left, center, and right sides of the FOV
    Raw images and deconvolution results of neurons collected with the large FOV light-sheet microscopy. (a) Large FOV raw images, RRLB reconstruction results, and RL reconstruction results; (b) the reconstruction results of different deconvolution methods correspond to the orange dashed box located on the left side of the FOV in Fig.7 (a), partial enlarged images and corresponding normalized intensity profiles, with the profile position corresponding to the yellow dashed line in Fig.7 (b); (c) RRLB reconstruction results, raw image and RL reconstruction results, corresponding to the yellow dashed box located to the right side of the FOV in Fig.7 (a); (d) the reconstruction results of different deconvolution methods correspond to the blue dashed box in Fig.7 (c)
    • Table 1. PSNR of simulated dataset reconstruction results

      View table

      Table 1. PSNR of simulated dataset reconstruction results

      AreaCNNDWDNRLRRLBRLB
      MeanSDMeanSDMeanSDMeanSDMeanSD
      Total62.022.3462.482.1058.531.9364.283.3264.441.68
      Area 159.751.7461.261.8157.551.9060.451.6361.771.47
      Area 261.321.5661.701.8557.971.8162.921.5263.871.24
      Area 362.331.7962.032.0958.181.9065.292.2064.931.44
      Area 463.921.3663.841.7560.221.5266.081.8766.111.05
      Area 564.551.1763.811.8859.251.5468.341.4865.601.16
      Area 663.461.2362.901.5058.241.5666.991.0564.860.79
      Area 763.431.1363.151.5358.341.2967.141.4964.821.25
      Area 862.671.3762.561.9858.041.4865.921.7464.621.08
      Area 961.471.7563.442.3260.401.9761.992.1465.011.49
      Area 1060.861.8461.751.8958.301.8462.661.8664.291.29
      Area 1158.452.0760.812.2357.332.0359.282.0963.001.42
    • Table 2. NCC of simulated dataset reconstruction results

      View table

      Table 2. NCC of simulated dataset reconstruction results

      AreaCNNDWDNRLRRLBRLB
      MeanSDMeanSDMeanSDMeanSDMeanSD
      Total0.9330.0330.9640.0160.8700.0450.9560.0320.9630.016
      Area 10.8870.0180.9370.0110.7860.0340.9080.0140.9270.017
      Area 20.9270.0220.9520.0290.8410.0390.9480.0370.9580.015
      Area 30.9430.0120.9670.0050.8690.0290.9720.0030.9690.008
      Area 40.9470.0110.9690.0040.8930.0210.9690.0050.9690.006
      Area 50.9610.0110.9760.0040.8880.0260.9840.0030.9700.008
      Area 60.9610.0110.9770.0030.8940.0220.9830.0030.9720.007
      Area 70.9590.0090.9770.0040.8900.0250.9820.0020.9700.005
      Area 80.9530.0130.9700.0050.8740.0330.9770.0040.9670.010
      Area 90.9320.0100.9740.0090.9240.0150.9470.0070.9720.007
      Area 100.9230.0100.9610.0060.8730.0290.9510.0060.9650.008
      Area 110.8660.0220.9490.0080.8350.0310.8940.0170.9540.013
    Tools

    Get Citation

    Copy Citation Text

    Haoyang Wu, Xiaojun Zhao, Xiaoquan Yang. Large Field-of-View Light-Sheet Image Reconstruction Based on Model-Driven Deconvolutional Network[J]. Laser & Optoelectronics Progress, 2025, 62(2): 0237015

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category: Digital Image Processing

    Received: May. 11, 2024

    Accepted: Jun. 12, 2024

    Published Online: Jan. 3, 2025

    The Author Email:

    DOI:10.3788/LOP241256

    CSTR:32186.14.LOP241256

    Topics