Fig. 1. Schematic of dual laser fluorescence microscopy system

Fig. 2. Diagram of synchronous timing of optoporation experiment

Fig. 3. Baseline calibration result of membrane potential change

Fig. 4. Effect of number of gold nanoparticles on viability of gastric cancer cells

Fig. 5. Effect of di-4-ANEPPS concentration on viability of gastric cancer cells

Fig. 6. Results of gold nanoparticle-mediated cell optoporation. (a) PI staining result; (b) Calcein-AM staining result; (c) bright field cell image; (d) combination of PI and Calcein-AM staining images

Fig. 7. Detection of laser-induced changes in cell membrane potential without gold nanoparticles (control group) and with gold nanoparticles (experimental group). (a) Fluorescence image of control group; (b) image of cell membrane potential change after introducing pulse laser; (c) fluorescence image of experimental group; (d) image of cell membrane potential change after introducing pulse laser