Chinese Journal of Lasers, Volume. 44, Issue 10, 1007001(2017)

Scattering Characteristics of Leukocytes on Polarized Light and Relationship Between Scattering Characteristics and Cell Structure

Bu Min1, Hu Shuangshuang1, Tao Zhaohe1, Jin Weifeng2, and Wang Yawei1
Author Affiliations
  • 1[in Chinese]
  • 2[in Chinese]
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    Figures & Tables(13)
    (a) Dyed lymphocyte; (b) undyed mononuclear; (c) eccentric sphere model; (d) theoretical model of double layer eccentric sphere
    Scattering light intensity distributions of homogeneous ball with different polarization incidence modes. (a) Natural light; (b) vertical polarized light; (c) horizontal polarized light
    Logarithmic distribution of Müller matrix elements in different scattering models. (a) M11; (b) M12; (c) M33; (d) M34
    Scattering light intensity distributions at different polarization incidence modes
    Schematic of light scattering coordinate system
    Variation of scattering light intensity with scattering angle and azimuth angle
    Relative distribution of scattering light intensity at different polarization incidence modes
    Logarithmic distributions of scattering light intensity varying with scattering angle and ratio of nuclear to cytoplasmic. (a) Horizontal linearly polarized light is incident; (b) vertical linearly polarized light is incident; (c) ratio of Fig. 8(a) result to Fig. 8(b) result; (d) difference between Fig. 8(a) result and Fig. 8(b) result
    (a) Logarithmic distribution of scattering light intensity varying with cell radius for horizontal linearly polarized light incidence; (b) ratio of difference to sum
    (a) Logarithmic distribution of scattering light intensity varying with wavelength for horizontal linearly polarized light incidence; (b) ratio of difference to sum
    (a) Logarithmic distribution of scattering light intensity varying with relative refractive index for horizontal linearly polarized light incidence; (b) ratio of difference to sum; (c) maximum and minimum positions of ratio
    • Table 1. Parameters in scattering models of lymphocyte

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      Table 1. Parameters in scattering models of lymphocyte

      ModelParameter
      Sphere modelr=15 μm, mcell=1.36, msuspension=1.3
      Eccentric sphere modelRcell=15 μm, rnucleus=7.5 μm, t=0.5, mcell=1.36, mnucleus=1.44, msuspension=1.3,φ=30°, γ=0.6667, H=5 μm
    • Table 2. Comparison of numerical results with VirtualLab simulation results

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      Table 2. Comparison of numerical results with VirtualLab simulation results

      Scatteringangle /(°)GOAVirtualLabRelativeerror /%Scatteringangle /(°)GOAVirtualLabRelativeerror /%
      01101803.690×10-73.510×10-75.14
      54.301×10-44.250×10-41.211752.787×10-72.672×10-74.32
      104.664×10-44.588×10-41.651704.820×10-74.590×10-75.01
      155.433×10-45.339×10-41.771653.855×10-73.714×10-73.81
      208.247×10-48.123×10-41.531602.012×10-71.944×10-73.50
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    Bu Min, Hu Shuangshuang, Tao Zhaohe, Jin Weifeng, Wang Yawei. Scattering Characteristics of Leukocytes on Polarized Light and Relationship Between Scattering Characteristics and Cell Structure[J]. Chinese Journal of Lasers, 2017, 44(10): 1007001

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    Paper Information

    Category: biomedical photonics and laser medicine

    Received: Apr. 7, 2017

    Accepted: --

    Published Online: Oct. 18, 2017

    The Author Email:

    DOI:10.3788/CJL201744.1007001

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