Fig. 1. Signal generation interface of the fluorescence fluctuation super-resolution comprehensive image reconstruction platform

Fig. 2. Comparison of fluorescence fluctuation characteristics between simulated and experimental data. (a)‒(c) Wide-field images of 100-frame simulated data, 100-frame experimental data, and 500-frame experimental data; (d)‒(f) time-dependent average intensity curves for three selected regions from wide-field images of 100-frame simulated data, 100-frame experimental data, and 500-frame experimental data, where ROI 1, ROI 2, and ROI 3 correspond to the boxed regions in yellow, green, and blue, respectively; (g)‒(i) intensity curves over time for the region ROI 3

Fig. 3. Fluorescence fluctuation super-resolution imaging and the intensity curves. (a) Simulation image operation interface; (b) experimental image operation interface; (c) normalized intensity distribution curves at the simulated microtubule crossing points for the wide-field image and super-resolution images reconstructed by 4 algorithms

Fig. 4. Multilayer perceptron architecture and its loss function curve. (a) Schematic diagram of the multilayer perceptron network structure established in this study; (b) error variation curve of the loss function

Fig. 5. Diagram of the multilayer perceptron model output results. (a) Parameters of input neurons in the multilayer perceptron model; (b) output results of the MLP model; (c) reconstructed images from four super-resolution algorithms and their corresponding CEF values