[1] [1] Vogelstein B, Kinzler K W. Digital PCR[J]. Proceedings of the National Academy of Sciences,1999,96（16）:9236—9241.

[2] [2] Bartlett J M S, Stirling D. A short history of the polymerase chain reaction[J]. Methods in Molecular Biology （Clifton, NJ）,2003,226:3—6.

[3] [3] Quan P L, Sauzade M, Brouzes E. dPCR: A technology review[J]. Sensors,2018,18（4）:1271.

[4] [4] Taylor S C, Laperriere G, Germain H. Droplet digital PCR versus qPCR for gene expression analysis with low abundant targets: From variable nonsense to publication quality data[J].Scientific Reports,2017,7（1）:2409.

[5] [5] Ren M S, Lin H, Chen S J, et al. Detection of pseudorabies virus by duplex droplet digital PCR assay [J]. Journal of Veterinary Diagnostic Investigation,2018,30（1）:105—112.

[6] [6] Niu M C, Cao W J, Wang Y C, et al. Droplet-based transcriptome profiling of individual synapses [J]. Nature Biotechnology,2023,41（9）:1332.

[7] [7] Takeshita T, Iwase H. dPCR mutational analyses in cell-free DNA: A comparison with tissues[J]. Methods in Molecular Biology,2019,1909:105—118.

[8] [8] Yi K, Wang X, Filippov S K, Zhang H. Emerging ctDNA detection strategies in clinical cancer theranostics [J]. Smart Medicine,2023,2（4）:e20230031.

[9] [9] Chin N A, Salihah N T, Shivanand P, Ahmed M U. Recent trends and developments of PCR-based methods for the detection of food-borne Salmonella bacteria and Norovirus[J]. Journal of Food Science and Technology,2022,59（12）:4570—4582.

[10] [10] Ottesen E A, Hong J W, Quake S R, Leadbetter J R. Microfluidic digital PCR enables multigene analysis of individual environmental bacteria[J]. Science,2006,314（5804）:1464—1467.

[11] [11] Morneau D. Droplet-based microfluidics[J]. Nature Reviews Methods Primers,2023,3（1）:32

[12] [12] Zhang P F, Abate A R. High-definition single-cell printing:Cell-by-cell fabrication of biological structures[J]. Advanced Materials,2020,32（52）:e2005346.

[13] [13] Beer N R, Wheeler E K, Lee-Houghton L, et al. On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets[J]. Analytical Chemistry,2008,80（6）:1854—1858.

[14] [14] Clark I C, Mudvari P, Thaploo S, et al. HIV silencing and cell survival signatures in infected T cell reservoirs[J]. Nature,2023,614（7947）:318—325.

[15] [15] Sun A C, Steyer D J, Allen A R, et al. A droplet microfluidic platform for high-throughput photochemical reaction discovery[J]. Nature Communications,2020,11（1）:6202.

[16] [16] Jacobs B K, Goetghebeur E, Clement L. Impact of variance components on reliability of absolute quantification using digital PCR [J]. BMC Bioinformatics,2014,15（1）:283.

[17] [17] Sidstedt M, Romsos EL, Hedell R, et al. Accurate digital polymorase chain reaction quantification of challenging samples applying inhibitor-toberant DNA pdymerases[J]. Analytical Chemistry,2017,89（3）:1642—1649.

[21] [21] Zhou S, Gou T, Hu J, et al. A highly integrated real-time digital PCR device for accurate DNA quantitative analysis [J]. Biosensors and Bioelectronics,2019,128:151—158.

[25] [25] Koo S B N, Kim Y S, Park C Y, Lee D J. Compact camera fluorescence detector for parallel-light lens-based real-time PCR system[J]. Sensors,2022,22（21）:8575.

[27] [27] Zi J, Bi H. Fluorescence microscope light source based on integrated LED [J]. Light: Science & Applications,2023,12（1）:224.

[28] [28] Li C, Kang N, Ye S, et al. All-in-one OsciDrop digital PCR system for automated and highly multiplexed molecular diagnostics [J]. Advanced Science,2024,11（21）:e2309557.

[29] [29] Gaov M, Wang X, Yan Z, et al. Temperature non-uniformity detection on dPCR chips and temperature sensor calibration [J]. RSC Advances,2022,12（4）:2375—2382.

[34] [34] Poojar P, Oiye I E, Aggarwal K, et al. Repeatability of image quality in very low-field MRI[J]. NMR in Biomedicine,2024,37（10）: e5198.

[35] [35] Liu H, Nan L, Chen F, et al. Functions and applications of artificial intelligence in droplet microfluidics[J]. Lab on a Chip,2023,23（11）:2497—2513.

[36] [36] Gardner K, Uddin M M, Tran L, et al. Deep learning detector for high precision monitoring of cell encapsulation statistics in microfluidic droplets[J]. Lab on a Chip,2022,22（21）:4067—4080.

[37] [37] Nan L, Zhang H D, Weitz D A, Shum H C. Development and future of droplet microfluidics[J]. Lab on a Chip,2024,24（5）:1135—1153.

[38] [38] Hess D, Dockalova V, Kokkonen P, et al. Exploring mechanism of enzyme catalysis by on-chip transient kinetics coupled with global data analysis and molecular modeling[J]. Chem,2021,7（4）:1066—1079.