Fig. 1. Schematic of dual wavelength measurement method

Fig. 2. Schematic of monocyte with spherical nucleus

Fig. 3. Phase distributions of monocyte with spherical nucleus. (a) Phase diagram for incidence along Z direction (λ₁＝632．8 nm); (b) phase diagram for incidence along Z direction (λ₂＝532 nm); (c) gradient diagram of Fig. 3 (a)

Fig. 4. Phase separation results. (a) Cytoplasmic phase; (b) nucleus phase

Fig. 5. Substructure phase separation of monocyte with horseshoe-shaped nucleus. (a) Three-dimensional model of cell; (b) spherical cytoplasmic phase; (c) horseshoe-shaped nucleus phase

Fig. 6. Eosinophil model. (a) Three-dimensional model; (b) phase diagram for incidence along Z direction; (c) gradient map; (d) cytoplasmic phase; (e) larger nucleus phase; (f) smaller nucleus phase

Fig. 7. Phase distributions of eosinophil model for incidence along the other direction. (a) Phase diagram for incidence along Y direction; (b) cytoplasmic phase; (c) phase of two nucleuses

Fig. 8. Phase distributions of monocyte model at orthogonal incidence (λ₁＝632.8 nm). (a) Phase diagram for incidence along Z direction; (b) phase diagram for incidence along Y direction; (c) gradient diagram of Fig. 8 (b)

Fig. 9. Three-dimensional reconstruction morphology of monocyte model with spherical nucleus

Fig. 10. Flow chart for reconstructing three-dimensional morphology of phase volume by orthogonal dual wavelength measurement method

Fig. 11. Morphological reconstruction of monocyte model with horseshoe-shaped nucleus. (a) Phase diagram in orthogonal direction (λ₁＝632.8 nm); (b) reconstructed three-dimensional morphology

Fig. 12. Eosinophil model under orthogonal dual-wavelength incidence. (a) Phase diagram for incidence along Z direction; (b) phase diagram for incidence along Y direction; (c) gradient diagram of Fig.12(b); (d) reconstructed three-dimensional morphology