[1] Shepard H M, Phillips G L, Thanos C D et al. Developments in therapy with monoclonal antibodies and related proteins[J]. Clinical Medicine, 17, 220-232(2017).

[2] Nelson P N, Reynolds G M, Waldron E E et al. Monoclonal antibodies[J]. Molecular Pathology, 53, 111-117(2000).

[3] Hamers-Casterman C, Atarhouch T, Muyldermans S et al. Naturally occurring antibodies devoid of light chains[J]. Nature, 363, 446-448(1993).

[4] Sroga P, Safronetz D, Stein D R. Nanobodies: a new approach for the diagnosis and treatment of viral infectious diseases[J]. Future Virology, 15, 195-205(2020).

[5] Yu X Y, Xu Q L, Wu Y et al. Nanobodies derived from Camelids represent versatile biomolecules for biomedical applications[J]. Biomaterials Science, 8, 3559-3573(2020).

[6] Smolarek D, Bertrand O, Czerwinski M. Variable fragments of heavy chain antibodies (VHHs): a new magic bullet molecule of medicine?[J]. Postepy Higieny i Medycyny Doswiadczalnej (Online), 66, 348-358(2012).

[7] Revets H, de Baetselier P, Muyldermans S. Nanobodies as novel agents for cancer therapy[J]. Expert Opinion on Biological Therapy, 5, 111-124(2005).

[8] Ladenson R C, Crimmins D L, Landt Y et al. Isolation and characterization of a thermally stable recombinant anti-caffeine heavy-chain antibody fragment[J]. Analytical Chemistry, 78, 4501-4508(2006).

[9] de Vos J, Devoogdt N, Lahoutte T et al. Camelid single-domain antibody-fragment engineering for (pre)clinical in vivo molecular imaging applications: adjusting the bullet to its target[J]. Expert Opinion on Biological Therapy, 13, 1149-1160(2013).

[10] Dumoulin M, Conrath K, van Meirhaeghe A et al. Single-domain antibody fragments with high conformational stability[J]. Protein Science, 11, 500-515(2002).

[11] van der Linden R H, Frenken L G, de Geus B et al. Comparison of physical chemical properties of llama VHH antibody fragments and mouse monoclonal antibodies[J]. Biochimica et Biophysica Acta, 1431, 37-46(1999).

[12] Hassanzadeh-Ghassabeh G, Devoogdt N, de Pauw P et al. Nanobodies and their potential applications[J]. Nanomedicine, 8, 1013-1026(2013).

[13] Vincke C, Loris R, Saerens D et al. General strategy to humanize a camelid single-domain antibody and identification of a universal humanized nanobody scaffold[J]. The Journal of Biological Chemistry, 284, 3273-3284(2009).

[14] Xavier C, Vaneycken I, D’Huyvetter Met al. Synthesis, preclinical validation, dosimetry, and toxicity of 68Ga-NOTA-anti-HER2 Nanobodies for iPET imaging of HER2 receptor expression in cancer[J]. Journal of Nuclear Medicine: Official Publication, 54, 776-784(2013).

[15] Vosjan M J W D, Perk L R, Roovers R C et al. Facile labelling of an anti-epidermal growth factor receptor Nanobody with 68Ga via a novel bifunctional desferal chelate for immuno-PET[J]. European Journal of Nuclear Medicine and Molecular Imaging, 38, 753-763(2011).

[16] Harmsen M M, de Haard H J. Properties, production, and applications of camelid single-domain antibody fragments[J]. Applied Microbiology and Biotechnology, 77, 13-22(2007).

[17] Arbabi-Ghahroudi M, Tanha J, MacKenzie R. Prokaryotic expression of antibodies[J]. Cancer Metastasis Reviews, 24, 501-519(2005).

[18] Frenken L G, Hessing J G, van den Hondel C A et al. Recent advances in the large-scale production of antibody fragments using lower eukaryotic microorganisms[J]. Research in Immunology, 149, 589-599(1998).

[19] van Bockstaele F, Holz J B, Revets H. The development of nanobodies for therapeutic applications[J]. Current Opinion in Investigational Drugs, 10, 1212-1224(2009).

[20] Muyldermans S. Nanobodies: natural single-domain antibodies[J]. Annual Review of Biochemistry, 82, 775-797(2013).

[21] Wei W J, Rosenkrans Z T, Liu J J et al. ImmunoPET: concept, design, and applications[J]. Chemical Reviews, 120, 3787-3851(2020).

[22] Debie P, Devoogdt N, Hernot S. Targeted nanobody-based molecular tracers for nuclear imaging and image-guided surgery[J]. Antibodies, 8, 12(2019).

[23] Traenkle B, Rothbauer U. Under the microscope: single-domain antibodies for live-cell imaging and super-resolution microscopy[J]. Frontiers in Immunology, 8, 1030(2017).

[24] Keppler A, Gendreizig S, Gronemeyer T et al. A general method for the covalent labeling of fusion proteins with small molecules in vivo[J]. Nature Biotechnology, 21, 86-89(2003).

[25] Gautier A, Juillerat A, Heinis C et al. An engineered protein tag for multiprotein labeling in living cells[J]. Chemistry & Biology, 15, 128-136(2008).

[26] Los G V, Encell L P, McDougall M G et al. HaloTag: a novel protein labeling technology for cell imaging and protein analysis[J]. ACS Chemical Biology, 3, 373-382(2008).

[27] Giepmans B N G, Adams S R, Ellisman M H et al. The fluorescent toolbox for assessing protein location and function[J]. Science, 312, 217-224(2006).

[28] Tsien R Y. The green fluorescent protein[J]. Annual Review of Biochemistry, 67, 509-544(1998).

[29] Snapp E L. Fluorescent proteins: a cell biologist’s user guide[J]. Trends in Cell Biology, 19, 649-655(2009).

[30] Hosein R E, Williams S A, Haye K et al. Expression of GFP-actin leads to failure of nuclear elongation and cytokinesis in Tetrahymena thermophila[J]. The Journal of Eukaryotic Microbiology, 50, 403-408(2003).

[31] Stadler C, Rexhepaj E, Singan V R et al. Immunofluorescence and fluorescent-protein tagging show high correlation for protein localization in mammalian cells[J]. Nature Methods, 10, 315-323(2013).

[32] Rothbauer U, Zolghadr K, Tillib S et al. Targeting and tracing antigens in live cells with fluorescent nanobodies[J]. Nature Methods, 3, 887-889(2006).

[33] Schiavon C R, Zhang T, Zhao B et al. Actin chromobody imaging reveals sub-organellar actin dynamics[J]. Nature Methods, 17, 917-921(2020).

[34] Plessner M, Melak M, Chinchilla P et al. Nuclear F-actin formation and reorganization upon cell spreading[J]. The Journal of Biological Chemistry, 290, 11209-11216(2015).

[35] Maier J L, Traenkle B, Rothbauer U. Real-time analysis of epithelial-mesenchymal transition using fluorescent single-domain antibodies[J]. Scientific Reports, 5, 13402(2015).

[36] O’Shea J M, Goutou A, Brydon J et al. Generation of photocaged nanobodies for intracellular applications in an animal using genetic code expansion and computationally guided protein engineering[J]. Chembiochem, 23, e202200321(2022).

[37] Ewers H. Nano resolution optical imaging through localization microscopy[M]. Cellular imaging techniques for neuroscience and beyond, 81-100(2012).

[38] Massa S, Vikani N, Betti C et al. Sortase A-mediated site-specific labeling of camelid single-domain antibody-fragments: a versatile strategy for multiple molecular imaging modalities[J]. Contrast Media & Molecular Imaging, 11, 328-339(2016).

[39] Debie P, van Quathem J, Hansen I et al. Effect of dye and conjugation chemistry on the biodistribution profile of near-infrared-labeled nanobodies as tracers for image-guided surgery[J]. Molecular Pharmaceutics, 14, 1145-1153(2017).

[40] Ries J, Kaplan C, Platonova E et al. A simple, versatile method for GFP-based super-resolution microscopy via nanobodies[J]. Nature Methods, 9, 582-584(2012).

[41] Virant D, Traenkle B, Maier J L et al. A peptide tag-specific nanobody enables high-quality labeling for dSTORM imaging[J]. Nature Communications, 9, 930(2018).

[42] Götzke H, Kilisch M, Martínez-Carranza M et al. The ALFA-tag is a highly versatile tool for nanobody-based bioscience applications[J]. Nature Communications, 10, 4403(2019).

[43] Schneider A F L, Benz L S, Lehmann M et al. Cell-permeable nanobodies allow dual-color super-resolution microscopy in untransfected living cells[J]. Angewandte Chemie (International Ed. in English), 60, 22075-22080(2021).

[44] Queiroz Zetune Villa Real K, Mougios N, Rehm R et al. A versatile synaptotagmin-1 nanobody provides perturbation-free live synaptic imaging and low linkage-error in super-resolution microscopy[J]. Small Methods, 7, e2300218(2023).

[45] Reinhardt S C M, Masullo L A, Baudrexel I et al. Ångström-resolution fluorescence microscopy[J]. Nature, 617, 711-716(2023).

[46] Hernot S, van Manen L, Debie P et al. Latest developments in molecular tracers for fluorescence image-guided cancer surgery[J]. The Lancet: Oncology, 20, e354-e367(2019).

[47] Prantner A M, Yin C, Kamat K et al. Molecular imaging of mesothelin-expressing ovarian cancer with a human and mouse cross-reactive nanobody[J]. Molecular Pharmaceutics, 15, 1403-1411(2018).

[48] van Manen L, Handgraaf H J M, Diana M et al. A practical guide for the use of indocyanine green and methylene blue in fluorescence-guided abdominal surgery[J]. Journal of Surgical Oncology, 118, 283-300(2018).

[49] Lwin T M, Hernot S, Hollandsworth H et al. Tumor-specific near-infrared nanobody probe rapidly labels tumors in an orthotopic mouse model of pancreatic cancer[J]. Surgery, 168, 85-91(2020).

[50] Guo X Y, Li C J, Jia X H et al. NIR-II fluorescence imaging-guided colorectal cancer surgery targeting CEACAM5 by a nanobody[J]. EBioMedicine, 89, 104476(2023).

[51] van Manen L, de Muynck L D A N, Baart V M et al. Near-infrared fluorescence imaging of pancreatic cancer using a fluorescently labelled anti-CEA nanobody probe: a preclinical study[J]. Biomolecules, 13, 618(2023).

[52] Choi H S, Gibbs S L, Lee J H et al. Targeted zwitterionic near-infrared fluorophores for improved optical imaging[J]. Nature Biotechnology, 31, 148-153(2013).

[53] Jiang Y X, Lin Y, Krishnaswamy S et al. Single-domain antibody-based non-invasive in vivo imaging of α-synuclein or tau pathology[J]. Science Advances, 9, eadf3775(2023).

[54] Jailkhani N, Ingram J R, Rashidian M et al. Noninvasive imaging of tumor progression, metastasis, and fibrosis using a nanobody targeting the extracellular matrix[J]. Proceedings of the National Academy of Sciences of the United States of America, 116, 14181-14190(2019).

[55] Wei W J, Zhang D, Wang C et al. Annotating CD38 expression in multiple myeloma with [18F]F-Nb1053[J]. Molecular Pharmaceutics, 19, 3502-3510(2022).

[56] Wang C, Chen Y M, Hou Y N et al. ImmunoPET imaging of multiple myeloma with [68Ga]Ga-NOTA-Nb1053[J]. European Journal of Nuclear Medicine and Molecular Imaging, 48, 2749-2760(2021).

[57] Zhang Y, Zhang D, An S X et al. Development and characterization of nanobody-derived CD47 theranostic pairs in solid tumors[J]. Research, 6, 0077(2023).

[58] An S X, Zhang D, Zhang Y et al. GPC3-targeted immunoPET imaging of hepatocellular carcinomas[J]. European Journal of Nuclear Medicine and Molecular Imaging, 49, 2682-2692(2022).

[59] Zhou Z Y, McDougald D, Devoogdt N et al. Labeling single domain antibody fragments with fluorine-18 using 2, 3, 5, 6-tetrafluorophenyl 6-[18F] fluoronicotinate resulting in high tumor-to-kidney ratios[J]. Molecular Pharmaceutics, 16, 214-226(2019).

[60] Zhang Y Y, Ding Y, Li N et al. Noninvasive imaging of tumor PD-L1 expression using [99mTc]Tc-labeled KN035 with SPECT/CT[J]. Molecular Pharmaceutics, 20, 690-700(2023).

[61] Zhao L Z, Liu C C, Xing Y et al. Development of a 99mTc-labeled single-domain antibody for SPECT/CT assessment of HER2 expression in breast cancer[J]. Molecular Pharmaceutics, 18, 3616-3622(2021).

[62] Witte M D, Wu T F, Guimaraes C P et al. Site-specific protein modification using immobilized sortase in batch and continuous-flow systems[J]. Nature Protocols, 10, 508-516(2015).

[63] Rashidian M, Wang L, Edens J G et al. Enzyme-mediated modification of single-domain antibodies for imaging modalities with different characteristics[J]. Angewandte Chemie (International Ed. in English), 55, 528-533(2016).

[64] Hebbrecht T, Liu J, Zwaenepoel O et al. Nanobody click chemistry for convenient site-specific fluorescent labelling, single step immunocytochemistry and delivery into living cells by photoporation and live cell imaging[J]. New Biotechnology, 59, 33-43(2020).

[65] Liu J H, Yang Y Q, Ma R et al. Research progress of organic NIR-Ⅱ fluorescent probes[J]. Chinese Journal of Lasers, 50, 2107101(2023).

[66] Jiang T, Gong H, Luo Q M et al. Whole-brain optical imaging[J]. Chinese Journal of Lasers, 50, 0307101(2023).

[67] Deng S L, Chen H B. Organic polymer dots and their bio-optical applications[J]. Chinese Journal of Lasers, 50, 1507401(2023).