Fig. 1. Light field microscopic imaging process

Fig. 2. Generation process of dataset

Fig. 3. Particle luminescence diameter under 10× objective lens

Fig. 4. Samples with different particle concentrations in dataset. (a)(b) 0.4; (c)(d) 0.8; (e)(f) 1.2

Fig. 5. Convolutional neural network structure for reconstruction of three-dimensional distribution of light field micro-particle

Fig. 6. Change of loss function with training process

Fig. 7. Schematic diagram of full width at half maxima

Fig. 8. Spatial distribution of particles and single particle intensity distribution (dashed line) at 0.4 concentration. (a) Theoretical distribution of particles;(b) particle distribution reconstructed by LFD method; (c) particle distribution reconstructed by the prediction model; (d) particle intensity distribution along the xdirection; (e) particle intensity distribution along the zdirection

Fig. 9. Spatial distribution of particles and single particle intensity distribution (dashed line) at 0.8 concentration. (a) Theoretical distribution of particles; (b) particle distribution reconstructed by LFD method; (c) particle distribution reconstructed by the prediction model;(d) particle intensity distribution along the xdirection; (e) particle intensity distribution along the zdirection

Fig. 10. Spatial distribution of particles and single particle intensity distribution (dashed line) at 1.2 concentration. (a) Theoretical distribution of particles; (b) particle distribution reconstructed by LFD method; (c) particle distribution reconstructed by the prediction model; (d) particle intensity distribution along the xdirection; (e) particle intensity distribution along the zdirection

Fig. 11. Reconstruction quality at different concentrations

Fig. 12. Particle extraction rate at different concentrations

Fig. 13. Particle distributions reconstructed by the prediction model and LFD for light field images with different R_SN

Fig. 14. Reconstruction quality of prediction model and LFD for light field images with different R_SN

Fig. 15. Reconstruction quality of the prediction model for light field images with different particle number with R_SN =10

Fig. 16. Experimental setup for microscale flow measurement

Fig. 17. Experimental light field image and reconstructed particle distribution. (a) Light field image; (b) particle distribution reconstructed by LFD; (c) particle distribution reconstructed by the prediction model; (d) comparison of particle intensity distribution along x direction; (e) comparison of particle intensity distribution along z direction

Fig. 18. Three-dimensional velocity field obtained by the prediction model

Fig. 19. Comparisons of the measured and theoretical velocity at z=50 μm