Infrared and Laser Engineering, Volume. 51, Issue 11, 20220735(2022)

Discussion on spatial resolution of microscopic imaging system (invited)

Shipei Dang1,2, Runze Li1, Meiling Zhou1, Jia Qian1, Dan Dan1, Xianghua Yu1, and Baoli Yao1,2
Author Affiliations
  • 1State Key Laboratory of Transient Optics and Photonics, Xi'an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences, Xi'an 710119, China
  • 2University of Chinese Academy of Sciences, Beijing 100049, China
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    Figures & Tables(8)
    Superposition intensity distributions formed by two diffracted incoherent point sources vs their separated distance. The blue (I1) and red (I2) lines represent the intensity distribution profiles of the two-point sources on the image plane, while the green one (Isum) represents total intensity distribution profile
    Superposition total intensity distribution profiles formed by two diffracted coherent point sources with different phase difference at the separated distance of Rayleigh criterion. (a) Total intensity distribution profiles of the superimposed two Airy disk against their phase difference and the distance; (b) Intensity distribution profiles at φ = 0, π/2 andπ, respectively
    Criterion of resolution limit for two point sources with the same phase (φ=0) under coherent illumination. (a)-(d) Superposition total intensity distributin profiles under different separated distances of the two point sources
    Criterion of resolution limit for two point sources with the opposite phase (φ = π) under coherent light illumination. (a)-(d) Superposition total intensity distribution profiles with the reducing separated distance dx of the two point sources; (e) Superposition intensity distributions profiles for different values of dx; (f) Respectively normalized intensity distributions profiles corresponding to (e)
    Diffraction intensity patterns of (a) a single point and (b) a single slit, and (c) their corresponding intensity distributiong profiles
    Focusing beyond the diffraction limit. (a) Airy disk distribution of single point diffraction focusing; (b) Diffraction focusing Airy pattern of three coherent point source when placing two points of P−1 and P+1 at dx=5.136 with the same intensity and π phase shift to the central point ofP0; (c)-(d) Diffraction Airy disk patterns when increasing the ratio of intensities of the P−1 and P+1 points to the central point P0, β = I±1/I0; (e) The intensity distribution profiles along the dashed lines in (a)-(d)
    Focusing beyond the diffraction limit. (a)-(d) The diffraction patterns when the ±1 points move approaching to the center point; (e) The intensity distribution profiles along the dashed lines in (a)-(d)
    • Table 1. Values of coefficients γ in resolution equation at different cases

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      Table 1. Values of coefficients γ in resolution equation at different cases

      Resolution criterionTargetCoherent imagingIncoherent imaging
      AbbeDouble slit-0.5
      RayleighDouble point0.820.61
      Double slit0.710.5
      SparrowDouble point0.730.47
      Double slit0.660.42
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    Shipei Dang, Runze Li, Meiling Zhou, Jia Qian, Dan Dan, Xianghua Yu, Baoli Yao. Discussion on spatial resolution of microscopic imaging system (invited)[J]. Infrared and Laser Engineering, 2022, 51(11): 20220735

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    Paper Information

    Category: Special issue-Fluorescence microscopy: techniques and applications

    Received: Oct. 12, 2022

    Accepted: --

    Published Online: Feb. 9, 2023

    The Author Email:

    DOI:10.3788/IRLA20220735

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