Chinese Journal of Pharmaceuticals, Volume. 56, Issue 7, 937(2025)

In vitro Micronucleus Test Combined with FISH Technology for Detecting Aneuploidy-inducing Agents

CUI Wenteng1,2, ZHOU Changhui2, FANG Yali2,3, LU Yanxin2, and CHANG Yan1,2、*
Author Affiliations
  • 1China State Institute of Pharmaceutical Industry, Shanghai 201203
  • 2Shanghai InnoStar Bio-Tech Co., Ltd., Shanghai 200043
  • 3School of Pharmacy, Fudan University, Shanghai 201203
  • show less
    References(18)

    [1] [1] LYNCH A M, EASTMOND D, ELHAJOUJI A,et al. Targets and mechanisms of chemically induced aneuploidy. Part 1 of the report of the 2017 IWGT workgroup on assessing the risk of aneugens for carcinogenesis and hereditary diseases [J].Toxicol Environ Mutagen, 2019,847: 403025.

    [2] [2] SIEGEL J J, AMON A. New insights into the troubles of aneuploidy [J].Annu Rev Cell Dev Biol, 2012,28(1): 189-214.

    [3] [3] PACCHIEROTTI F, MASUMURA K, EASTMOND D A,et al. Chemically induced aneuploidy in germ cells. Part Ⅱ of the report of the 2017 IWGT workgroup on assessing the risk of aneugens for carcinogenesis and hereditary diseases[J].Mutat Res Genet Toxicol Environ Mutagen, 2019,848: 403023.

    [4] [4] WILLIAMS B R, AMON A. Aneuploidy: cancer's fatal flaw?[J].Cancer Res, 2009,69(13): 5289-5291.

    [6] [6] REICHSTEIN I S, KONIG M, WOJTYSIAK N,et al. Replacing animal-derived components inin vitrotest guidelines OECD 455 and 487 [J].Sci Total Environ, 2023,868: 161454.

    [7] [7] HEDDLE J A, CARRANO A V. The DNA content of micronuclei induced in mouse bone marrow by -irradiation: evidence that micronuclei arise from acentric chromosomal fragments [J].Mutat Res, 1977,44(1): 63-69.

    [8] [8] FENECH M. Thein vitromicronucleus technique [J].Mutat Res, 2000,455(1/2): 81-95.

    [9] [9] DECORDIER I, KIRSCH-VOLDERS M. Fluorescence in situ hybridization (FISH) technique for the micronucleus test[J].Genoto Asse, 2013,1: 237-244.

    [10] [10] KIRSCH-VOLDERS M, PLAS G, ELHAJOUJI A,et al. Thein vitroMN assay in 2011: origin and fate, biological significance, protocols, high throughput methodologies and toxicological relevance [J].Arch Toxicol, 2011,85: 873-899.

    [11] [11] CHUNG H W, KANG S J, KIM S Y. A combination of the micronucleus assay and a FISH technique for evaluation of the genotoxicity of 1, 2, 4-benzenetriol [J].Mutat Res, 2002,516(1/2): 49-56.

    [12] [12] NERSEYAN A, PERRONE E, ROGGIERI P,et al. Cytogenetic toxicity of cycloplatam in human lymphocytes: detection by the micronucleus test and fluorescence in situ hybridization [J].Anti-cancer Drugs, 2006,17(3): 289-295.

    [13] [13] HOVHANNISYAN G G. Fluorescence in situ hybridization in combination with the comet assay and micronucleus test in genetic toxicology [J].Mol Cytogenet, 2010,3(1): 17.

    [14] [14] ZHANG L S, HONMA M, HAYASHI M,et al. A comparative study of TK6 human lymphoblastoid and L5178Y mouse lymphoma cell lines in the in vitro micronucleus test[J].Mutat Res, 1995,347(3-4): 105-115.

    [15] [15] KIRKLAND D. Evaluation of different cytotoxic and cytostatic measures for thein vitromicronucleus test (MNVit): summary of results in the collaborative trial [J].Mutat Res, 2010,702(2): 139-147.

    [16] [16] KIRCHNER S, ZELLER A. Comparison of different cytotoxicity measures for thein vitromicronucleus test(MNVit) in L5178Y tk(+/-) cells: summary of 4 compounds(mitomycin C, cyclophosphamide, colchicine and diethylstilboestrol) with clastogenic and aneugenic mode of action [J].Mutat Res, 2010,702(2): 193-198.

    [17] [17] LORGE E, THTBAUD V, AARDEMA M J,et al. SFTG international collaborative study onin vitromicronucleus test Ⅰ. General conditions and overall conclusions of the study[J].Mutat Res, 2006,607(1): 13-36.

    [18] [18] JIE Y M, JIA C. Chromosomal composition of micronuclei in mouse NIH 3T3 cells treated with acrylamide, extract of Tripterygium hypoglaucum (level) hutch, mitomycin C and colchicine, detected by multicolor FISH with centromeric and telomeric DNA probes [J].Mutagenesis, 2001,16(2): 145-149.

    [19] [19] PARRY E M, PARRY J M, CORSO C,et al. Detection and characterization of mechanisms of action of aneugenic chemicals [J].Mutagenesis, 2002,17(6): 509-521.

    Tools

    Get Citation

    Copy Citation Text

    CUI Wenteng, ZHOU Changhui, FANG Yali, LU Yanxin, CHANG Yan. In vitro Micronucleus Test Combined with FISH Technology for Detecting Aneuploidy-inducing Agents[J]. Chinese Journal of Pharmaceuticals, 2025, 56(7): 937

    Download Citation

    EndNote(RIS)BibTexPlain Text
    Save article for my favorites
    Paper Information

    Category:

    Received: Feb. 18, 2025

    Accepted: Aug. 26, 2025

    Published Online: Aug. 26, 2025

    The Author Email: CHANG Yan (ychang@innostar.cn)

    DOI:10.16522/j.cnki.cjph.2025.07.013

    Topics