Chinese Journal of Lasers, Volume. 45, Issue 3, 307013(2018)
Fluorescent Microsphere Segmentation and Classification Based on Watershed and Semi-Supervised Minor Reconstruction Error
Figures & Tables(7)
Fig. 3. Results with improved watershed segmentation. (a1)(a2) Original images of the fluorescent microspheres; (b1)(b2) grayscale maps after semi-threshold foreground extraction; (c1)(c2) segmented results without improvement; (d1)(d2) gradient images; (e) final segmented results
Fig. 4. Statistical distribution of fluorescent microsphere objects in HSV color space. Statistical distributions of (a) hue channel; (b) saturation channel; (c) value channel
Fig. 5. Seven different types of fluorescent microsphere samples classes. (a) Class 1, (b) class 2, (c) class 3, (d) class 4, (e) class 5, (f) class 6, and (g) class 7
Table 1. Overall classification accuracies and run times of different methods with different labeled data samples per class
View tableTable 1. Overall classification accuracies and run times of different methods with different labeled data samples per class
Classification method 2 labels 4 labels 6 labels 8 labels Overall classification accuracy /% Run time /s Overall classification accuracy /% Run time /s Overall classification accuracy /% Run time /s Overall classification accuracy /% Run time /s LDA 70.0±0.066 0.087 76.4±0.044 0.123 79.4±0.022 0.135 81.1±0.027 0.135 KNN 77.2±0.064 0.004 81.0±0.039 0.005 82.0±0.023 0.006 82.4±0.028 0.007 RF 77.3±0.044 3.801 80.3±0.022 3.904 81.7±0.019 4.020 82.7±0.013 4.028 SRC 72.9±0.050 1.281 77.9±0.034 1.326 80.1±0.024 1.360 81.1±0.033 1.375 SVM 76.1±0.050 1.146 80.0±0.025 1.254 81.7±0.028 1.402 83.0±0.016 1.557 SSMREC 84.3±0.030 5.919 85.7±0.019 5.954 86.2±0.021 6.033 86.5±0.023 6.093
Table 2. Each class classification accuracies and overall accuracies in different methods with 2 labeled data samples per class%
View tableTable 2. Each class classification accuracies and overall accuracies in different methods with 2 labeled data samples per class%
Classification method Classification accuracy of each class Overall accuracy Class 1 Class 2 Class 3 Class 4 Class 5 Class 6 Class 7 LDA 73.0 84.7 69.2 65.3 56.8 73.4 69.0 69.8 KNN 77.2 92.4 85.9 65.0 66.1 79.6 77.1 77.2 RF 92.9 92.5 73.3 62.3 55.9 72.5 90.1 77.3 SRC 69.6 91.1 85.8 66.0 56.8 79.8 67.5 72.9 SVM 74.8 91.2 85.7 64.0 68.7 79.2 74.4 76.2 SSMREC 95.5 95.3 86.3 66.6 69.5 80.3 93.3 84.3
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Huang Hong, Jin Yingying, Li Zhengying, Duan Yule, Shi Guangyao. Fluorescent Microsphere Segmentation and Classification Based on Watershed and Semi-Supervised Minor Reconstruction Error[J]. Chinese Journal of Lasers, 2018, 45(3): 307013
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Received: Sep. 5, 2017
Accepted: --
Published Online: Mar. 6, 2018
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