To address the issue of handwriting alteration in the field of court scientific document examination, we employ reflection transformation imaging (RTI) technique to capture the three-dimensional characteristics of cross-strokes in handwritten characters. The proposed method allows us to examine cross-stroke sequences from the same type of ink across multiple modes. First, we create three types of samples: cross-crossing, tilt-crossing, and point-crossing, dividing each into two categories based on stroke order: “first horizontal then vertical” and “first vertical then horizontal”, with 50 samples in each category. Second, 1200 samples are imaged and analyzed using the RTI technique to evaluate the detection rates of various samples under different RTI modes. Analysis of variance (ANOVA) is then performed on the detection rates in normal visualization mode. Preliminary experimental results show that the ballpoint pens and neutral pens exhibit the best test effectiveness in cross-crossing and tilted-crossing samples, with detection rates of up to 95%. Show pens and fiber pens also perform well in these categories, with detection rates of 85% and 65% respectively. But their detection effect decreases in point-crossing samples, with a detection rate of only 70% for ballpoint pens and neutral pens, and 25% for Show pens and fiber pens. ANOVA reveals notable differences in detection rates between sample types in normal visualization mode. RTI technology offers new opportunities for enhancing document inspection methods and shows promising potential for future development in this field.