The role of human glycolipid transfer protein (GLTP) in initiation and progression of tumor and its epigenetic regulation mechanisms were analyzed by bioinformatics methods. The expression and survival analysis results of human GLTP in pancreatic adenocarcinoma (PAAD) tissues was significantly higher than that in adjacent normal tissues by using online bioinformatics analysis tools such as UCSC Genome Browser, EMBOSS and GEPIA. These results suggest that GLTP may be involved in the initiation and progression of PAAD. The analysis of GLTP expression level and pathological stage of PAAD patients showed that there was no significant difference in GLTP expression level among different pathological stages of PAAD patients. The results of methylation analysis showed that the methylation level of human GLTP was significantly correlated with the expression level of GLTP in PAAD cells. The results of histone modification analysis indicate that histone H3K4Me1 and H3K27Ac may affect initiation and progression of tumor by up-regulating the expression of human GLTP. The results of competing endogenous RNAs (ceRNA) regulation analysis show that human GLTP has potential effects on a variety of microRNAs (miRNAs), and these miRNAs are strongly associated with many tumors and signaling pathways. The results of this study provide a reference for the further study of the function and epigenetic regulation mechanisms of human GLTP.