Chinese Optics, Volume. 15, Issue 6, 1332(2022)

Multi-color virtual fluorescence emission difference microscopy

Yu-ran HUANG1, Zhi-min ZHANG2, Wan-jie DONG1, Liang XU1, Yu-bing HAN1, Xiang HAO1, Cui-fang KUANG1,3,4、*, and Xu LIU1
Author Affiliations
  • 1State Key Laboratory of Modern Optical Instrumentation, College of Optical Science and Engineering, Zhejiang University, Hangzhou 310027, China
  • 2Research Center for Intelligent Chips and Devices, Zhejiang Lab, Hangzhou 311121, China
  • 3Ningbo Research Institute, Zhejiang University, Ningbo 315100, China
  • 4Collaborative Innovation Center of Extreme Optics, Shanxi University, Taiyuan 030006, China
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    Figures & Tables(4)
    Sketch of virtual fluorescence emission difference microscopy
    Imaging model of the system. (a) The black, red, and blue curves are the normalized PSFs of the center, left and right edge channels in parallel detection, respectively; (b) the normalized PSFs of the edge channels after photon reassignment, matching the outer contour of the center channel; vFED imaging model without noise at (c) n=1, q=0.35, p=1 and (d) n=7, q=0. 5, p=0.85, the red, blue and black curves are virtual solid spot image PSF obtained by photon reassignment and summation, the hollow spot image PSF obtained from the center channel, and the vFED image PSF, respectively; (e) vFED imaging model when background noise is considered, the background noise contained in each channel accounts for the same proportion of the total number of photons in the channel, and the parameters n, q, p are the same as those in (c); (f) the blue, red, black, and green curves are the confocal PSF, solid spot photon reassignment method PSF, vFED image PSF calculated according to the n, q, p parameters of (c) and (d) , respectively. The background noise level is the same as that in (e)
    Fluorescent particle imaging results. (a) Confocal image; (b) solid spot photon reassignment image; (c) vFED image; (d) virtual solid spot image; (e) hollow spot image; (f) normalized intensity of the white truncation line in figures (a)~(c)
    Imaging results of three-color biological samples. (a) Solid spot photon reassignment image; (b) enlarged view of the area inside the box in (a); (c) vFED image of the same area
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    Yu-ran HUANG, Zhi-min ZHANG, Wan-jie DONG, Liang XU, Yu-bing HAN, Xiang HAO, Cui-fang KUANG, Xu LIU. Multi-color virtual fluorescence emission difference microscopy[J]. Chinese Optics, 2022, 15(6): 1332

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    Paper Information

    Category: Original Article

    Received: Apr. 24, 2022

    Accepted: --

    Published Online: Feb. 9, 2023

    The Author Email:

    DOI:10.37188/CO.2022-0080

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