Chinese Optics, Volume. 15, Issue 6, 1332(2022)
Multi-color virtual fluorescence emission difference microscopy
Fig. 1. Sketch of virtual fluorescence emission difference microscopy
Fig. 2. Imaging model of the system. (a) The black, red, and blue curves are the normalized PSFs of the center, left and right edge channels in parallel detection, respectively; (b) the normalized PSFs of the edge channels after photon reassignment, matching the outer contour of the center channel; vFED imaging model without noise at (c)
Fig. 3. Fluorescent particle imaging results. (a) Confocal image; (b) solid spot photon reassignment image; (c) vFED image; (d) virtual solid spot image; (e) hollow spot image; (f) normalized intensity of the white truncation line in figures (a)~(c)
Fig. 4. Imaging results of three-color biological samples. (a) Solid spot photon reassignment image; (b) enlarged view of the area inside the box in (a); (c) vFED image of the same area
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Yu-ran HUANG, Zhi-min ZHANG, Wan-jie DONG, Liang XU, Yu-bing HAN, Xiang HAO, Cui-fang KUANG, Xu LIU. Multi-color virtual fluorescence emission difference microscopy[J]. Chinese Optics, 2022, 15(6): 1332
Category: Original Article
Received: Apr. 24, 2022
Accepted: --
Published Online: Feb. 9, 2023
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