Laser & Optoelectronics Progress, Volume. 56, Issue 9, 092402(2019)

Detection of Interaction Between Peach-Gum Polysaccharides and Galectin-3 via Surface Plasmon Resonance Imaging

Yuhuan Shuai1, Pan Qi2, Ying Li3, Cuiying Hu1, Mengjie Cai4, Yanhong Ran4, Shiping Li5、**, and Jingang Zhong5、*
Author Affiliations
  • 1 Department of Physics, College of Science and Engineering, Jinan University, Guangzhou Guangdong, 510632, China
  • 2 Department of Electronics Engineering, Guangdong Communication Polytechnic, Guangzhou, Guangdong 510650, China
  • 3 Pre-University, Jinan University, Guangzhou, Guangdong 510610, China
  • 4 Department of Bioengineering, College of Life Science and Technology, Jinan University, Guangzhou, Guangdong 510632, China
  • 5 Department of Optoelectronic Engineering, College of Science and Engineering, Jinan University, Guangzhou, Guangdong 510632, China
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    Figures & Tables(14)
    Reflectivity and phase versus refractive index at incident angle of 70.58°
    Reflectivity and phase versus sample thickness at incident angle of 70.58°
    Experimental device of SPRI
    Physical map of experimental device
    Reflectivity and phase diagrams during water droplet evaporation. (a)(c)(e) Intensity diagrams; (b)(d)(f) phase diagrams
    Rreflectivity and phase versus time during water droplet evaporation
    Digital holograms, intensity images, and phase images recorded at 0 and 270 s. (a) Digital hologram at 0 s; (b) reconstructed intensity image at 0 s; (c) reconstructed phase image at 0 s; (d) three-dimensional (3D) representation of (b); (e) 3D representation of (c); (f) digital hologram at 270 s; (g) reconstructed intensity image at 270 s; (h) reconstructed phase image at 270 s; (i) 3D representation of (g); (j) 3D representation of (h)
    PGP-1 phase versus time
    Standard curve of PGP-1 reaction
    Calculated curve of binding rate for PGP-1 reaction
    PGP-2 phase versus time
    Standard curve of PGP-2 reaction
    Calculated curve of binding rate for PGP-2 reaction
    • Table 1. Kinetic parameters

      View table

      Table 1. Kinetic parameters

      SampleBinding rate constant,ka /(M-s-)Binding equilibriumconstant, KA /M-
      PGP-1PGP-26663.403930.258.36×1051.24×105
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    Yuhuan Shuai, Pan Qi, Ying Li, Cuiying Hu, Mengjie Cai, Yanhong Ran, Shiping Li, Jingang Zhong. Detection of Interaction Between Peach-Gum Polysaccharides and Galectin-3 via Surface Plasmon Resonance Imaging[J]. Laser & Optoelectronics Progress, 2019, 56(9): 092402

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    Paper Information

    Category: Optics at Surfaces

    Received: Oct. 16, 2018

    Accepted: Dec. 4, 2018

    Published Online: Jul. 5, 2019

    The Author Email: Shiping Li (tlisp@jnu.edu.cn), Jingang Zhong (tzjg@jnu.edu.cn)

    DOI:10.3788/LOP56.092402

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