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Laser & Optoelectronics Progress, Volume. 56, Issue 15, 151701(2019)

Raman Shift Characteristics of Substance Expression and Accumulation Structure in Lung Cancer Cells

Yu Yang1, Zhengrong Tao1, Jing Yan2, Shuxing Lü1, Ran Wang1, Xiaodan Liu1, and Xuguo Sun1、*
Author Affiliations
  • 1 School of Medical Laboratory, Tianjin Medical University, Tianjin 300203, China
  • 2 School of Computer Science and Engineering, Tianjin University of Technology, Tianjin 300384, China
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    AbstractGet PDF(in Chinese)
    Figures&Tables (7)
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    References (16)
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    Figures & Tables(7)
    Trends in cell culture days and cell viability

    Fig. 1. Trends in cell culture days and cell viability

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    Morphological characteristics of A549 and Met-5A cells in optical microscopy with extension of culture time. (a) A549; (b) Met-5A

    Fig. 2. Morphological characteristics of A549 and Met-5A cells in optical microscopy with extension of culture time. (a) A549; (b) Met-5A

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    Optical images of A549 and Met-5A cells under Raman instruments. (a) A549; (b) Met-5A

    Fig. 3. Optical images of A549 and Met-5A cells under Raman instruments. (a) A549; (b) Met-5A

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    Comparison of Raman shift characteristics between A549 and Met-5A cells. Solid arrow indicates Raman peak at 1082 cm-1, and dotted arrow indicates Raman peak at 1155 cm-1

    Fig. 4. Comparison of Raman shift characteristics between A549 and Met-5A cells. Solid arrow indicates Raman peak at 1082 cm-1, and dotted arrow indicates Raman peak at 1155 cm-1

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    Staining results of A549 and Met-5A cells with PAP and H&E, respectively. (a)(b)(e)(f) A549; (c)(d)(g)(h) Met-5A

    Fig. 5. Staining results of A549 and Met-5A cells with PAP and H&E, respectively. (a)(b)(e)(f) A549; (c)(d)(g)(h) Met-5A

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    • Table 1. Characteristic peak assignments for Raman spectra

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      Table 1. Characteristic peak assignments for Raman spectra

      Peak position /cm-1MajorassignmentsPAPstain affinityH&E stainaffinity
      786DNA:O-P-O++
      1082O-P-O- stretch++
      1155C-N stretching (protein)+++
      1258Amide III--
      1304CH2 deformation (lipid)--
      1450CH2CH3 twistingmode(lipid)--
      1658Amide I (C=O stretchingmode of proteins,a-helix conformation)--
      Note: + and++ indicate affinity degree of H&E and PAP dyes with corresponding chemical groups; - indicates that H&E and PAP dyes are negatively colored with corresponding chemical groups.

    • Table 2. Image characteristic parameters of A549 and Met-5A cells stained by two dyes

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      Table 2. Image characteristic parameters of A549 and Met-5A cells stained by two dyes

      Characteristic parameterCellPAP (n=100)H&E (n=100)
      Energy varianceA5497.91419±1.665257.98664±1.68421
      Met-5A4.62469±1.375118.16944±1.83352
      t10.771-0.519
      p<0.0010.605
      Color meanA5497.06792±0.048927.11570±0.04107
      Met-5A7.10321±0.036027.05825±0.02611
      t-4.1098.347
      p<0.001<0.001
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    Yu Yang, Zhengrong Tao, Jing Yan, Shuxing Lü, Ran Wang, Xiaodan Liu, Xuguo Sun. Raman Shift Characteristics of Substance Expression and Accumulation Structure in Lung Cancer Cells[J]. Laser & Optoelectronics Progress, 2019, 56(15): 151701

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    Paper Information

    Category: Medical Optics and Biotechnology

    Received: Jan. 25, 2019

    Accepted: Mar. 6, 2019

    Published Online: Aug. 5, 2019

    The Author Email: Xuguo Sun (sunxuguo@tmu.edu.cn)

    DOI:10.3788/LOP56.151701

    Topics

    laser devices and laser physics
    Lasers and Laser Optics
    Laser physics
    laser manufacturing
    Instrumentation, Measurement and Metrology