Experimental Investigation of Wide-Range Detection of Live Algal Cell Density Based on Variable Fluorescence Statistical Analysis

Xie Wang; Gaofang Yin; Nanjing Zhao; Xiang Hu; Peng Huang; Mingjun Ma; Min Xu; Tingting Gan; Ruifang Yang; Li Fang; Xiaoling Zhang; Xiang Wang; Zhao Cheng

doi:10.3788/AOS231817

Acta Optica Sinica, Volume. 44, Issue 12, 1201017(2024)

Experimental Investigation of Wide-Range Detection of Live Algal Cell Density Based on Variable Fluorescence Statistical Analysis

Xie Wang^1,2, Gaofang Yin^1,2、*, Nanjing Zhao^1,2、**, Xiang Hu², Peng Huang², Mingjun Ma², Min Xu^1,2, Tingting Gan^1,2, Ruifang Yang², Li Fang², Xiaoling Zhang³, Xiang Wang⁴, and Zhao Cheng²

Author Affiliations

¹University of Science and Technology of China, Hefei 230026, Anhui , China

²Key Laboratory of Environment Optics and Technology, Anhui Institute of Optics and Fine Mechanics, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui , China

³Information Materials and Intelligent Sensing Laboratory of Anhui Province, Institute of Material Science and Information Technology, Anhui University, Hefei 230601, Anhui , China

⁴School of Electrical Engineering, Anhui Polytechnic University, Wuhu 241200, Anhui , China

show less

Abstract Get PDF(in Chinese)

Figures & Tables(10)

Fig. 1. Distribution of mean F_v of subsamples with changes algal density

Download full size

Fig. 2. Schematic diagram of viable algal cell detcection system

Download full size

Fig. 3. Relation curves between variance σ²of different algal cells’ mean F_v and interval gain coefficient a. (a) Chlorella pyrenoidesa; (b) Selenastrum capricornutum; (c) Scenedesmus obliqnus; (d) Dunaliella salina

Download full size

Fig. 4. Relation curve between variance σ² of algal cells’ mean F_v and interval gain coefficient a

Download full size

Fig. 5. Comparison of test results of three detection methods

Download full size

Fig. 6. Comparison results of density calculated by microscopic analysis method and the proposed method. (a) Chlorella pyrenoidesa; (b) Selenastrum capricornutum; (c) Scenedesmus obliqnus; (d) Dunaliella salina

Download full size

Fig. 7. Comparison between microscopic detection and system calculated densities of mixed algal samples. (a) High density of natural water; (b) high density of VFSA; (c) low density

Download full size

Table 1. Test datas between variance σ²of different algal cells’ mean F_vand interval gain coefficient a

View table

Table 1. Test datas between variance σ²of different algal cells’ mean F_vand interval gain coefficient a

Algal specie	Algal cell density /（cells·mL^-1）	Distribution interval of F_v	Subsample number	Interval gain coefficient a	σ²
Chlorellapyrenoidesa	2317.07	0-0.8	0	0.14	0.005
		0.8-1.2	458
		1.2-2.0	22
	231.72	0-0.8	67	0.12	0.040
		0.8-1.2	339
		1.2-2.0	74
	23.17	0-0.8	141	0.08	0.135
		0.8-1.2	214
		1.2-2.0	125
Selenastrum capricornutum	1546.94	0-0.8	3	0.13	0.007
		0.8-1.2	473
		1.2-2.0	4
	232.04	0-0.8	60	0.11	0.033
		0.8-1.2	356
		1.2-2.0	64
	23.21	0-0.8	144	0.07	0.135
		0.8-1.2	214
		1.2-2.0	122
Scenedesmusobliqnus	1989.88	0-0.8	3	0.15	0.007
		0.8-1.2	371
		1.2-2.0	6
	198.99	0-0.8	75	0.11	0.039
		0.8-1.2	331
		1.2-2.0	74
	19.90	0-0.8	148	0.07	0.138
		0.8-1.2	212
		1.2-2.0	120
Dunaliellasalina	1923.10	0-0.8	2	0.14	0.007
		0.8-1.2	476
		1.2-2.0	2
	192.31	0-0.8	57	0.10	0.031
		0.8-1.2	361
		1.2-2.0	62
	19.23	0-0.8	125	0.06	0.090
		0.8-1.2	239
		1.2-2.0	116

Table 2. Comparison test results of three methods

View table

Table 2. Comparison test results of three methods

Dilution ratio	method /（cells·mL^-1） Microscopic analysis	VFSA method /（cells·mL^-1）	ARE /%	This method /（cells·mL^-1）	ARE /%
1	4640.82	855.54	81.56	5005.38	7.86
3	1540.61	297.08	80.80	1828.17	18.18
5	817.75	170.85	81.59	930.54	0.26
10	433.36	103.05	77.79	532.51	14.75
50	92.82	31.25	66.33	92.18	0.39
100	46.41	22.16	52.25	37.48	19.24
200	24.60	18.70	19.13	25.64	10.47
400	12.61	10.71	15.07	10.87	13.80

Table 3. Comparison of microscopic detection and system calculated densities in mixed algal sample

View table

Table 3. Comparison of microscopic detection and system calculated densities in mixed algal sample

Sample solution	Density ofPeridiniumumbonatum /（cells·mL^-1）	Density ofSelenastrumcapricornutum /（cells·mL^-1）	Mixing proportion of Peridinium umbonatum and Selenastrum capricornutum	Microscopic analysis method /（cells·mL^-1）	method /（cells·mL^-1） This	ARE /%
High density ofnatural water	1030.79	1884.22	1∶4	1708.14	1669.34	2.27
			1∶1	1372.16	1429.92	4.21
			4∶1	1201.47	1127.88	6.12
High density ofVFSA	113.49	132.13	1∶4	128.40	132.13	2.90
			1∶1	122.82	133.54	8.73
			4∶1	117.22	119.94	2.32
Low density	8.41	14.21	1∶4	13.05	12.83	1.69
			1∶1	11.31	12.39	9.55
			4∶1	9.57	8.41	12.12

Tools

Get Citation

Copy Citation Text

Xie Wang, Gaofang Yin, Nanjing Zhao, Xiang Hu, Peng Huang, Mingjun Ma, Min Xu, Tingting Gan, Ruifang Yang, Li Fang, Xiaoling Zhang, Xiang Wang, Zhao Cheng. Experimental Investigation of Wide-Range Detection of Live Algal Cell Density Based on Variable Fluorescence Statistical Analysis[J]. Acta Optica Sinica, 2024, 44(12): 1201017

Download Citation

EndNote(RIS)BibTex Plain Text

Set citation alerts for article

Save article for my favorites

Paper Information

Category: Atmospheric Optics and Oceanic Optics

Received: Nov. 21, 2023

Accepted: Jan. 2, 2024

Published Online: Jun. 3, 2024

The Author Email: Yin Gaofang (gfyin@aiofm.ac.cn), Zhao Nanjing (njzhao@aiofm.ac.cn)

DOI:10.3788/AOS231817

CSTR:32393.14.AOS231817

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Test datas between variance σ2of different algal cells’ mean Fvand interval gain coefficient a

Table 1. Test datas between variance σ2of different algal cells’ mean Fvand interval gain coefficient a

Table 2. Comparison test results of three methods

Table 2. Comparison test results of three methods

Table 3. Comparison of microscopic detection and system calculated densities in mixed algal sample

Table 3. Comparison of microscopic detection and system calculated densities in mixed algal sample

Table 1. Test datas between variance σ²of different algal cells’ mean F_vand interval gain coefficient a

Table 1. Test datas between variance σ²of different algal cells’ mean F_vand interval gain coefficient a