Unlike ensemble-averaging measurements, single-molecule tracking provides quantitative information on the kinetics of individual molecules within living cells in real time and may provide insight into the respective molecular interactions behind that. The advancement of single-molecule tracking has been significantly boosted by the development of high-resolution microscopy techniques. In this review, we will discuss this aspect with a particular focus on their recent advance in MINFLUX nanoscopy with feedback approaches where tracking is performed in real time. MINFLUX localization requires fewer than 100 photons from a ～1 nm-sized fluorophore, enabling precise tracking. This approach, which demands over an order of magnitude fewer photons than other localization-based techniques (such as STORM, PLAM), allows molecular tracking with single-digit nanometer accuracy in less than 1ms — an achievement previously unattainable.