Fig. 1. Overview of scanning light-field microscopy (sLFM) and its applications. (a) In sLFM^[1,2], a 2D scanning module and a microlens array capture high-resolution spatial–angular data at near-diffraction-limited resolution^[2,9]. (b) After scanning, a time-weighted algorithm and mutual iterative tomography with digital adaptive optics (DAO)^[2,13] enable pixel realignment and aberration correction, yielding a volumetric reconstruction. (c) These advancements support diverse biomedical applications, such as visualizing migrasome dynamics in mouse liver vasculature (DAOSLIMIT)^[2], high-speed vascular imaging in zebrafish (MiSLFM)^[11], and large-scale neural activity mapping in awake mice (csLFM)^[12].