Acta Optica Sinica, Volume. 45, Issue 5, 0530001(2025)

Parallel Acquisition Method for Quasi-3D Fluorescence Spectra Based on Software Lock-In Amplification Technique

Qian Li1, Junyu Wu1, Huanhuan Huang2, Qingsheng Xue1, Diansheng Cao1, Fupeng Wang1, and Xiaoning Luan1、*
Author Affiliations
  • 1College of Physics and Optoelectronic Engineering, Faculty of Information Science and Engineering, Ocean University of China, Qingdao 266100, Shandong , China
  • 2Shandong Provincial Lunan Geology and Exploration Institute (Shandong Provincial Bureau of Geology and Mineral Resources No.2 Geological Brigade), Jining 272000, Shandong , China
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    Figures & Tables(11)
    Flow chart of quasi-3D fluorescence spectrum parallel acquisition method based on software lock-in amplification technique
    Schematic diagram of light path of laser induced fluorescent lidar
    Prototype of laser induced fluorescence lidar
    Lock-in amplification principle
    Software lock-in amplification system based on Simulink
    Fluorescence spectra of planktic algae directly collected. (a) Sunlight background; (b) dark background
    Results of phase locking amplification of Chlorella fluorescence excited by different wavelengths under sunlight. (a) 405 nm; (b) 465 nm; (c) 520 nm; (d) 638 nm
    Fluorescence spectra of planktic algae excited by different wavelengths after lock-in amplification under sunlight background. (a) 405 nm; (b) 465 nm; (c) 520 nm; (d) 638 nm
    Normalized results of fluorescence spectra of different algae. (a) Aps; (b) Aigp; (c) Acv; (d) Ancm
    • Table 1. Algae species for experiments

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      Table 1. Algae species for experiments

      PhylumAlgaeAbbreviation
      ChlorophytaChlorella vulgarisAcv
      ChlorophytaPlatymonas subcordataAps
      BacillariophytaNitzschia closterium minutissimaAncm
      ChrysophytaIsochrysis galbana parkeAigp
    • Table 2. Main technical parameters of receiving telescope

      View table

      Table 2. Main technical parameters of receiving telescope

      Technical parameterParameter value
      Aperture of primary mirror /mm105
      Field of view /(°)3
      Focal length /mm800
      Dimension /(mm×mm)111×144.5
      Weight /g946.4
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    Qian Li, Junyu Wu, Huanhuan Huang, Qingsheng Xue, Diansheng Cao, Fupeng Wang, Xiaoning Luan. Parallel Acquisition Method for Quasi-3D Fluorescence Spectra Based on Software Lock-In Amplification Technique[J]. Acta Optica Sinica, 2025, 45(5): 0530001

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    Paper Information

    Category: Spectroscopy

    Received: Dec. 2, 2024

    Accepted: Dec. 28, 2024

    Published Online: Mar. 21, 2025

    The Author Email: Xiaoning Luan (luanxiaoning@ouc.edu.cn)

    DOI:10.3788/AOS241825

    CSTR:32393.14.AOS241825

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