Since playing an indispensable role in both the application and mechanism of superconductivity, superconducting films are the important link between them. Pulsed laser deposition (PLD) is one of the most commonly used techniques for growing superconducting films. In this article, the research progress on superconducting films including cuprate, iron-based, nitride and titanium oxide films by PLD is reviewed, and two developed PLD methods like superconducting long-tape and large-area-film techniques for the practical application of high-Tc superconductors are introduced. Additionally, an advanced film preparation method for high-throughput combinatorial films based on genetic engineering is recently utilized for the research of high-Tc superconductivity. Further development of the novel high-throughput superconductivity research mode will be successfully employed for building high-dimensional phase diagrams, exploring more key quantitative laws and then understanding the underlying mechanism of high-Tc superconductivity.