Experiment Science and Technology, Volume. 21, Issue 5, 38(2023)

Comprehensive Experimental Design of Immobilized Dual-enzymes in Sodium Alginate Hydrogel Beads for Rapid Detection of Glucose

Yanhong LIU1, Na WANG2, and Jing LI1、*
Author Affiliations
  • 1Experiment Teaching Center of Basic Chemistry, Sichuan University, Chengdu 610064, China
  • 2College of Chemistry, Sichuan University, Chengdu 610064, China
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    References(11)

    [2] CAO X Y, GUO Y M, ZHAO M et al. An efficient multi-enzyme cascade platform based on mesoporous metal-organic frameworks for the detection of organophosphorus and glucose[J]. Food Chemistry, 381, 132282(2020).

    [3] BILAL M, HUSSAIN N, AMERICO-PINHEIRO J H P et al. Multi-enzyme co-immobilized nano-assemblies: Bringing enzymes together for expanding bio-catalysis scope to meet biotechnological challenges[J]. International Journal of Biological Macromolecules, 186, 735-749(2021).

    [4] CLAAßEN C, GERLACH T, ROTHERA D. Stimulus-responsive regulation of enzyme activity for one-step and multi-step syntheses[J]. Advanced Synthsis Cataysis, 361, 2387-2401(2019).

    [5] ZHANG H, HUA S F, ZHANG L. Co-immobilization of cellulase and glucose oxidase on graphene oxide by covalent bonds: A biocatalytic system for one-pot conversion of gluconic acid from carboxymethyl cellulose[J]. Journal of Chemical Technology & Biotechnology, 95, 1116-1125(2020).

    [6] DHAMECHA D, MOVSAS R, SANO U et al. Applications of alginate microspheres in therapeutics delivery and cell culture: Past, present and future[J]. International Journal of Pharmaceutics, 569, 118627(2019).

    [7] COLEMAN R J, LAWRIE G, LAMBERT L K et al. Phosphorylation of alginate: Synthesis, characterization, and evaluation of in vitro mineralization capacity[J]. Biomacromolecules, 12, 889-897(2011).

    [8] LEE K Y, MOONEY D. Alginate: Properties and biomedical applications[J]. Journal of Polymer Science, 37, 106-126(2012).

    [9] CUADROS T R, SKURTYS O, AGUILERA J M. Mechanical properties of calcium alginate fibers produced with a microfluidic device[J]. Carbohydrate Polymers, 89, 1198-1206(2012).

    [10] JOHNSON K A, MUZZIN N, TOUFANIAN S et al. Drug-impregnated, pressurized gas expanded liquid-processed alginate hydrogel scaffolds for accelerated burn wound healing[J]. Acta Biomaterial, 112, 101-111(2020).

    [11] GUPTA A, KOWLCZUK M, HEASELGRAVE W et al. The production and application of hydrogels for wound management: A review[J]. European Polymer Journal, 111, 134-151(2019).

    [14] XU S, ZHANG Y, ZHU Y et al. Facile one-step fabrica-tion of glucose oxidase loaded polymeric nanoparticles decorating MWCNTs for constructing glucose biosensing platform: Structure matters[J]. Biosensors and Bioelectronics, 135, 153-159(2019).

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    Yanhong LIU, Na WANG, Jing LI. Comprehensive Experimental Design of Immobilized Dual-enzymes in Sodium Alginate Hydrogel Beads for Rapid Detection of Glucose[J]. Experiment Science and Technology, 2023, 21(5): 38

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    Paper Information

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    Received: Mar. 15, 2022

    Accepted: --

    Published Online: Nov. 28, 2023

    The Author Email:

    DOI:10.12179/1672-4550.20220146

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