Fig. 1. The principle of the STED super-resolution microscope

Fig. 2. The organic fluorescent probes for STED super-resolution imaging of lipid droplets

Fig. 3. The applications of lipid droplets fluorescent probe LAQ1 in multicolor confocal imaging and STED super-resolution imaging. (a) Three-color fluorescence imaging of lipid droplets, endoplasmic reticulum and mitochondria; (b) signal intensity profiles across the white arrow; (c) STED imaging of lipid droplets; (d) confocal and STED imaging signal intensity profiles across the same lipid droplet^[19]

Fig. 4. The applications of lipid droplets fluorescent probe DTPA-BT-M in (a) STED super-resolution and (b) two-photon imaging^[20]

Fig. 5. STED super-resolution imaging of lipid droplets fluorescent probe Lipi-DSB. (a) STED laser power-dependent resolution; (b) two-color STED imaging of Lipi-DSB paired with mitochondria fluorescent probe tetramethylrhodamine; (c) time-lapse STED super-resolution imaging (1000 consecutive frames); (d) dynamic tracking of nascent lipid droplet fusion processes at the nanoscale by STED super-resolution imaging^[21]

Fig. 6. Confocal and STED super-resolution imaging of the lipid droplets fluorescent probe Lipi-BDTO. (a) Comparison of confocal and STED imaging; (b) 3D STED imaging^[22]

Fig. 7. The principle of SIM super-resolution microscope^[64]

Fig. 8. The organic fluorescent probes for SIM super-resolution imaging of lipid droplets

Fig. 9. SIM super-resolution imaging of the lipid droplets fluorescent probe LD-FG. (a) Buffer strategy enables stable imaging of lipid droplets; (b) fluorescence switching property of the fluorescent probe; (c) coalescence between adjacent lipid droplets; (d) coalescence between non-adjacent lipid droplets^[27]

Fig. 10. Applications of organic fluorescent probes in SIM super-resolution imaging of lipid droplets. (a) Fluorescent probe DTZ-TPA-DCN used for staining nuclear lipid droplets^[28]; (b) fluorescent probe NIM-3A used for analyzing the number and size of lipid droplets^[29]; (c) fluorescent probe NIM-7 used for tracking dynamic processes of lipid droplets and lysosomes^[30]

Fig. 11. Schematic diagram of molecular localization technology^[68]

Fig. 12. Applications of light-switchable fluorescent probes in PALM super-resolution imaging of lipid droplets. (a) The organic fluorescent probes with fluorescence ON/OFF characteristics; (b) bright field and two-color imaging of lipid droplets in adipocyte under wide-field and PALM microscopes^[31]