Flat-top beam illumination for polarization-sensitive second-harmonic generation microscopy

Bing Wang; Xiang Li; Wenhui Yu; Binglin Shen; Rui Hu; Junle Qu; Liwei Liu

doi:10.3788/COL202422.061701

Chinese Optics Letters, Volume. 22, Issue 6, 061701(2024)

Flat-top beam illumination for polarization-sensitive second-harmonic generation microscopy

Bing Wang^1,2, Xiang Li^1,2, Wenhui Yu^1,2, Binglin Shen^1,2, Rui Hu^1,2, Junle Qu^1,2, and Liwei Liu^1,2、*

Author Affiliations

¹State Key Laboratory of Radio Frequency Heterogeneous Integration, Shenzhen University, Shenzhen 518060, China

²Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China

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References(28)

[1] C. Odin, Y. Le Grand, A. Renault et al. Orientation fields of nonlinear biological fibrils by second harmonic generation microscopy. J. Microsc., 229, 32(2008).

[2] F. Tiaho, G. Recher, D. Rouède. Estimation of helical angles of myosin and collagen by second harmonic generation imaging microscopy. Opt. Express, 15, 12286(2007).

[3] R. A. R. Rao, M. R. Mehta, K. C. Toussaint. Fourier transform-second-harmonic generation imaging of biological tissues. Opt. Express, 17, 14534(2009).

[4] C. L. Hsieh, Y. Pu, R. Grange et al. Digital phase conjugation of second harmonic radiation emitted by nanoparticles in turbid media. Opt. Express, 18, 12283(2010).

[5] C. L. Hsieh, Y. Pu, R. Grange et al. Imaging through turbid layers by scanning the phase conjugated second harmonic radiation from a nanoparticle. Opt. Express, 18, 20723(2010).

[6] G. Recher, D. Rouède, P. Richard et al. Three distinct sarcomeric patterns of skeletal muscle revealed by SHG and TPEF microscopy. Opt. Express, 17, 19763(2009).

[7] X. Chen, O. Nadiarynkh, S. Plotnikov et al. Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure. Nat. Protoc., 7, 654(2012).

[8] A. Golaraei, K. Mirsanaye, Y. Ro et al. Collagen chirality and three-dimensional orientation studied with polarimetric second-harmonic generation microscopy. J. Biophotonics, 12, e201800241(2019).

[9] R. Ambekar, T.-Y. Lau, M. Walsh et al. Quantifying collagen structure in breast biopsies using second-harmonic generation imaging. Biomed. Opt. Express, 3, 2021(2012).

[10] K. Tilbury, C.-H. Lien, S.-J. Chen et al. Differentiation of Col I and Col III isoforms in stromal models of ovarian cancer by analysis of second harmonic generation polarization and emission directionality. Biophys. J., 106, 354(2014).

[11] K. R. Campbell, R. Chaudhary, J. M. Handel et al. Polarization-resolved second harmonic generation imaging of human ovarian cancer. J. Biomed. Opt., 23, 066501(2018).

[12] M. D. Miler, M. Rabasovic, A. J. Aleksic et al. Polarization-resolved SHG imaging as a fast screening method for collagen alterations during aging: comparison with light and electron microscopy. J. Biophotonics, 14, e202000362(2021).

[13] V.-H. Le, S. Lee, B. Kim et al. Correlation between polarization sensitive optical coherence tomography and second harmonic generation microscopy in skin. Biomed. Opt. Express, 6, 2542(2015).

[14] M. Samim, N. Prent, D. Dicenzo et al. Second harmonic generation polarization properties of myofilaments. J. Biomed. Opt., 19, 056005(2014).

[15] B. C. Syverud, M.-A. Mycek, L. M. Larkin. Quantitative, label-free evaluation of tissue-engineered skeletal muscle through multiphoton microscopy. Tissue Eng. Part C Methods, 23, 616(2017).

[16] H. Zhao, R. Cisek, A. Karunendiran et al. Live imaging of contracting muscles with wide-field second harmonic generation microscopy using a high power laser. Biomed. Opt. Express, 10, 5130(2019).

[17] V. Pal, C. Tradonsky, R. Chriki et al. Generating flat-top beams with extended depth of focus. Appl. Opt., 57, 4583(2018).

[18] Q. Long, G. Peng, Y. Chen et al. Centimeter-scale low-damage micromachining on single-crystal 4H–SiC substrates using a femtosecond laser with square-shaped flat-top focus spots. Ceram. Int., 47, 23134(2021).

[19] F. A. W. Coumans, E. van der Pol, L. W. M. M. Terstappen. Flat-top illumination profile in an epifluorescence microscope by dual microlens arrays. Cytometry A, 81, 324(2012).

[20] B. Khaw, J. Croop, A. Tang et al. Flat-field illumination for quantitative fluorescence imaging. Opt. Express, 26, 15276(2018).

[21] C. J. Rowlands, F. Ströhl, P. P. V. Ramirez et al. Flat-field super-resolution localization microscopy with a low-cost refractive beam-shaping element. Sci. Rep., 8, 5630(2018).

[22] M. M. Kabir, H. S. Rajput, V. A. Kelkar et al. Demonstration of flat-top beam illumination in widefield multiphoton microscopy. J. Biomed. Opt., 25, 014503(2020).

[23] A. Möhl, S. Kaldun, C. Kunz et al. Tailored focal beam shaping and its application in laser material processing. J. Laser Appl., 31, 042019(2019).

[24] P. Stoller, K. M. Reiser, P. M. Celliers et al. Polarization-modulated second harmonic generation in collagen. Biophys. J., 82, 3330(2002).

[25] S. W. Chu, S. Y. Chen, G. W. Chern et al. Studies of χ(2)/χ(3) tensors in submicron-scaled bio-tissues by polarization harmonics optical microscopy. Biophys. J., 86, 3914(2004).

[26] D. G. Winters, D. R. Smith, P. Schlup et al. Measurement of orientation and susceptibility ratios using a polarization-resolved second-harmonic generation holographic microscope. Biomed. Opt. Express, 3, 2004(2012).

[27] S. V. Plotnikov, A. C. Millard, P. J. Campagnola et al. Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres. Biophys. J., 90, 693(2006).

[28] C. Odin, T. Guilbert, A. Alkilani et al. Collagen and myosin characterization by orientation field second harmonic microscopy. Opt. Express, 16, 16151(2008).

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Bing Wang, Xiang Li, Wenhui Yu, Binglin Shen, Rui Hu, Junle Qu, Liwei Liu, "Flat-top beam illumination for polarization-sensitive second-harmonic generation microscopy," Chin. Opt. Lett. 22, 061701 (2024)

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Paper Information

Category: Biophotonics

Received: Jan. 22, 2024

Accepted: Feb. 27, 2024

Published Online: Jun. 18, 2024

The Author Email: Liwei Liu (liulw@szu.edu.cn)

DOI:10.3788/COL202422.061701

CSTR:32184.14.COL202422.061701

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology