In this paper, the centimeter-scale uniform silver nanoparticles (AgNPs) arrays with controllable sizes are prepared by using anodic aluminum oxide template-assisted vacuum thermal evaporation technology. Then, the monolayer MoS2/AgNPs array hybrid structures are obtained by wet transfer method. The surface-enhanced fluorescence (SEF) properties of the hybrid structure are systematically investigated. We find that the fabricated AgNPs arrays could be used as efficient SEF substrates to enhance the fluorescence intensity of monolayer MoS2. Meanwhile, the fluorescence intensity of monolayer MoS2 can been further improved with temperature decreasing. This study may provide a basis for the application of MoS2 in the field of optoelectronic integration.