Chinese Journal of Lasers, Volume. 45, Issue 2, 207016(2018)
Super-Resolution Localization Microscopy with Scientific Complementary Metal Oxide Semiconductor Camera
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Fig. 1. Principle of super-resolution localization microscopy. (a) Airy disk from single molecule fluorescence imaging; (b) localized single molecule position; (c) separation of densely labelled fluorescence molecules into multiple frames; (d) single molecule fluorescence image from conventional fluorescence microscopy; (e) image from super-resolution localization microscopy
Fig. 2. Optical setup for super-resolution localization microscopy based on fiber combiner
Table 1. Main parameters of several typical low light detectors
View tableTable 1. Main parameters of several typical low light detectors
Camera Pixel Pixel size /μm Quantum efficiency /% Full frame rate / (frame·s-1) Read noise /e Extra noise factor Estimated price iXon 897 (Andor) 512×512 16 95 35 < 1 $35000 Neo (Andor) 2560×2160 6.5 57 100 1 1 $20000 Flash 4.0 (Hamamatsu) 2048×2048 6.5 72 100 1.3 1 $20000
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Gui Dan, Shang Mingtao, Huang Zhenli. Super-Resolution Localization Microscopy with Scientific Complementary Metal Oxide Semiconductor Camera[J]. Chinese Journal of Lasers, 2018, 45(2): 207016
Paper Information
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Received: Oct. 8, 2017
Accepted: --
Published Online: Feb. 28, 2018
The Author Email: Zhenli Huang (leo@hust.edu.cn)
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