Utilizing Three-Dimensional Fluorescence Spectra and Parallel Factor Analysis Algorithm to Quantify Concentration of Multiple Metabolic Fluorophores in a Cell Culture Medium

Xiaokang Song; Qiang Zhao; Yuanzhi Zhang; Yikun Wang; Guoqing Deng; Ling Zhu

doi:10.3788/CJL202249.0911001

Chinese Journal of Lasers, Volume. 49, Issue 9, 0911001(2022)

Utilizing Three-Dimensional Fluorescence Spectra and Parallel Factor Analysis Algorithm to Quantify Concentration of Multiple Metabolic Fluorophores in a Cell Culture Medium

Xiaokang Song^1,2, Qiang Zhao^1,3、*, Yuanzhi Zhang², Yikun Wang², Guoqing Deng², and Ling Zhu^2、**

Author Affiliations

¹School of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, Anhui, China

²Anhui Institute of Optics and Fine Mechanics, Hefei Institute of Physical Science, Chinese Academy of Sciences, Hefei 230031, Anhui, China

³Anhui Provincial Key Laboratory of Environmental Pollution Control and Resource Reuse, Anhui Jianzhu University, Hefei 230601, Anhui, China

show less

Abstract Get PDF(in Chinese)

Figures & Tables(11)

Fig. 1. Standardizing process procedure of raw dataset

Download full size

Fig. 2. Fluorescence emission and excitation matrix spectra based on 3 to 5 components configuration

Download full size

Fig. 3. High leverage data identification from 3 dimensions within the cubic matrix

Download full size

Fig. 4. Core consistency value of three and four components models. (a) Three components; (b) four components

Download full size

Fig. 5. Three-dimensional contour maps of four components (VB6, FAD, TRY, and NADH) after modeling

Download full size

Fig. 6. Excitation spectra after split-half analysis (S4C6T3)

Download full size

Fig. 7. Emission spectra after split-half analysis (S4C6T3)

Download full size

Fig. 8. Relationship between fluorescence intensity and concentration of TRY, NADH, FAD and VB6

Download full size

Table 1. Levels of orthogonal test and concentration of TRY, NADH, FAD, and VB6

View table

Table 1. Levels of orthogonal test and concentration of TRY, NADH, FAD, and VB6

No.	Level	Mass concentration /(μg·mL^－1)
No.	Level	TRY	NADH	FAD	VB6
1	1111	0.12	20	2	1.65
2	2222	0.24	40	4	3.3
3	3333	0.48	80	8	6.6
4	4444	0.96	160	16	13.2
5	5555	1.92	320	32	26.4
6	2345	0.24	80	16	26.4
7	3451	0.48	160	32	1.65
8	4512	0.96	320	2	3.3
9	5123	1.92	20	4	6.6
10	1234	0.12	40	8	13.2
11	3524	0.48	320	4	13.2
12	4135	0.96	20	8	26.4
13	5241	1.92	40	16	1.65
14	1352	0.12	80	32	3.3
15	2413	0.24	160	2	6.6
16	4253	0.96	40	32	6.6
17	5314	1.92	80	2	13.2
18	1425	0.12	160	4	26.4
19	2531	0.24	320	8	1.65
20	3142	0.48	20	16	3.3
21	5432	1.92	160	8	3.3
22	1543	0.12	320	16	6.6
23	2154	0.24	20	32	13.2
24	3215	0.48	40	2	26.4
25	4321	0.96	80	4	1.65

Table 2. Predication mass concentration and recovery of TRY, NADH, FAD, and VB6 by PARAFAC

View table

Table 2. Predication mass concentration and recovery of TRY, NADH, FAD, and VB6 by PARAFAC

Fluorophore	Test sets	Added mass concentration /(μg·mL^－1)	Predicted mass concentration /(μg·mL^－1)	Recovery /%
TRY	T1	1.920	1.827	95.2
	T2	0.120	0.118	98.6
	T3	0.240	0.193	80.6
	T4	0.480	0.496	103.3
	T5	0.960	1.185	123.4
NADH	T1	160	230.428	144.0
	T2	320	279.800	87.4
	T3	20	29.673	148.3
	T4	40	37.255	93.1
	T5	80	51.249	64.1
FAD	T1	8	7.450	93.1
	T2	16	15.326	95.8
	T3	32	35.081	109.6
	T4	2	2.030	101.5
	T5	4	4.090	102.5
VB6	T1	3.3	2.941	89.1
	T2	6.6	6.813	103.2
	T3	13.2	13.741	104.1
	T4	26.4	27.479	104.0
	T5	1.65	1.60	97.1

Table 3. Precision of the algorithm obtained by using PARAFAC
View table
Table 3. Precision of the algorithm obtained by using PARAFAC
Fluorophore Average recovery /% RMSEP /(μg·mL^－1) ARE /% LOD /(μg·mL^－1)
TRY 100.2±15.5 0.124 13.216 0.013
NADH 107.4±37.1 43.312 36.937 52.628
FAD 100.5±6.4 1.601 6.112 0.003
VB6 99.5±6.5 0.639 6.331 0.012

Tools

Get Citation

Copy Citation Text

Xiaokang Song, Qiang Zhao, Yuanzhi Zhang, Yikun Wang, Guoqing Deng, Ling Zhu. Utilizing Three-Dimensional Fluorescence Spectra and Parallel Factor Analysis Algorithm to Quantify Concentration of Multiple Metabolic Fluorophores in a Cell Culture Medium[J]. Chinese Journal of Lasers, 2022, 49(9): 0911001

Download Citation

EndNote(RIS)BibTex Plain Text

Set citation alerts for article

Save article for my favorites