Fig. 1. (a) Schematic diagram of DDC-FT system; (b) simulational image of the composite phase plate and image of photolithography plate; (c) images of fluo-spheres; (d) calibration curves of DH-PSF rotating angle versus the depth of the sample for the 0th and the ±1st diffraction orders

Fig. 2. Localization precision calibration of DDC-FT system

Fig. 3. Track of the fluo-spheres. (a) Images captured at different time; (b) tracks of the fluo-spheres (the designed track is red and the captured track is blue); (c) zoom in the red box area in Fig.(b)

Fig. 4. Dynamic tracking of macrophage. (a) Profile of macrophage under white light illumination; (b) fluorescent image of macrophage; (c) dynamic tracking reconstruction of fluo-spheres; (d) zoom in the yellow box area in Fig.(c)