Fig. 1. Flow chart of a convenient nucleic acid detection method based on CRISPR/Cas12a

Fig. 2. Schematics of instrument structure. (a) Physical map of experimental instrument; (b) schematic of overall structure of testing instrument; (c) schematic of optical cassette structure; (d) schematic of instrument base structure

Fig. 3. Optical inspection in minimalist mode. (a) Workflow chart of nucleic acid detection APP; (b) real-time fluorescent CRISPR reaction time; (c) positive result showed by nucleic acid detection APP; (d) negative result showed by nucleic acid detection APP

Fig. 4. Schematics of optical circuit. (a) Schematic of fluorescence recognition circuit for small portable device; (b) schematic of coaxial optical fiber detection principle

Fig. 5. Experimental detection limit in minimalist mode. (a) Results showed by nucleic acid detection APP; (b) fluorescence data analysis diagram

Fig. 6. Specific analysis (data represented as the mean ± standard deviation of three experimental replicates). (a) Specific analysis of different virus; (b) base mismatch specificity analysis

Fig. 7. Detection results comparison between our method and PCR method ( negative reaction time is replaced by 0 min)