Polarization Characterization of Fluorescence Resonance Energy Transfer Optofluidic Lasers

Jiaqi Zhao; Tingting Zhang; Tianjun Zhang; Wenjie Wang; Shaoding Liu

doi:10.3788/LOP231079

Laser & Optoelectronics Progress, Volume. 61, Issue 9, 0914005(2024)

Polarization Characterization of Fluorescence Resonance Energy Transfer Optofluidic Lasers

Jiaqi Zhao, Tingting Zhang, Tianjun Zhang, Wenjie Wang^*, and Shaoding Liu

Key Laboratory of Advanced Transducers and Intelligent Control System of Education, Taiyuan University of Technology, Taiyuan 030024, Shanxi , China

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Figures & Tables(6)

Fig. 1. Experimental setup and materials. (a) Single-stranded DNA; (b) G-Quadruplex DNA; (c) diagram of the experimental setup

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Fig. 2. Spectral graphs. (a) Spectral graph of the receptor laser in parallel polarization direction at 0 $μ m o l / L$ K⁺; (b) spectral graph of the receptor laser in vertical polarization direction at 0 $μ m o l / L$ K⁺; (c) spectral graph of the receptor laser in parallel polarization direction at 10 $μ m o l / L$ K⁺; (d) spectral graph of the receptor laser in vertical polarization direction at 10 $μ m o l / L$ K⁺

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Fig. 3. Laser pumping threshold curves of TAMRA receptors in parallel and vertical polarization directions for different K⁺ concentrations at DNA molecule concentration 50 $μ m o l / L$ . (a) 0 $μ m o l / L$ ; (b) 5 $μ m o l / L$ ; (c) 10 $μ m o l / L$ ; (d) 20 $μ m o l / L$ ;(e) 50 $μ m o l / L$

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Fig. 4. Scatter plot of R_SER values of acceptor with potassium ion concentration

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Table 1. Specific parameters of the configured G-quadruplex solution
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Table 1. Specific parameters of the configured G-quadruplex solution
Ingredient Concentration pH
Tris-HCl buffer 0.1 $m o l / L$ 8.04
DNA molecule solution 50 $μ m o l / L$
KCl solution 0， 5， 10， 20， 50 $μ m o l / L$
LiCl solution 100， 95， 90， 80， 50 $μ m o l / L$

Table 2. Curve slopes and laser thresholds in the parallel and perpendicular directions for acceptor lasers with different concentration of K⁺ in the solvent

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Table 2. Curve slopes and laser thresholds in the parallel and perpendicular directions for acceptor lasers with different concentration of K⁺ in the solvent

c（K⁺） /（μmol/L）	Slope of the threshold curve /（mm²·μJ^-1）		Threshold /（μJ·mm^-2）
c（K⁺） /（μmol/L）	Parallel polarization	Vertical polarization	Parallel polarization	Vertical polarization
0	98.47	4.88	356.52	715.84
5	153.41	12.77	114.19	71.93
10	315.59	54.41	80.66	65.30
20	1022.50	524.47	16.41	14.67
50	869.69	565.54	10.82	11.52

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Jiaqi Zhao, Tingting Zhang, Tianjun Zhang, Wenjie Wang, Shaoding Liu. Polarization Characterization of Fluorescence Resonance Energy Transfer Optofluidic Lasers[J]. Laser & Optoelectronics Progress, 2024, 61(9): 0914005

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Paper Information

Category: Lasers and Laser Optics

Received: Apr. 13, 2023

Accepted: Jun. 1, 2023

Published Online: Apr. 29, 2024

The Author Email: Wenjie Wang (wangwenjie@tyut.edu.cn)

DOI:10.3788/LOP231079

CSTR:32186.14.LOP231079

Topics

laser devices and laser physics

Lasers and Laser Optics

Laser physics

laser manufacturing

Instrumentation, Measurement and Metrology

Table 1. Specific parameters of the configured G-quadruplex solution

Table 1. Specific parameters of the configured G-quadruplex solution

Table 2. Curve slopes and laser thresholds in the parallel and perpendicular directions for acceptor lasers with different concentration of K+ in the solvent

Table 2. Curve slopes and laser thresholds in the parallel and perpendicular directions for acceptor lasers with different concentration of K+ in the solvent

Table 2. Curve slopes and laser thresholds in the parallel and perpendicular directions for acceptor lasers with different concentration of K⁺ in the solvent

Table 2. Curve slopes and laser thresholds in the parallel and perpendicular directions for acceptor lasers with different concentration of K⁺ in the solvent