Fig. 1. Profiles and intensity distributions of Gaussian beams, Airy beams, and planar-Airy beams in different directions at static and scanning. (a) The y-z cross-section profile of static Gaussian beam at the beam waist (x=0); (b) The x-z cross-section profile of one-photon excited, Gaussian light-sheet scanned along the y-axis; (c) The x-z cross-section profile of Gaussian light-sheet excited by two-photon; (d) The z-direction intensity distribution of the Gaussian light-sheet excited by one-photon and two-photon at x = 0; (e)-(h) The profile and intensity distribution of Airy beam in different directions at static and after scanning; (i)-(l) The profile and intensity distribution of planar-Airy beam in different directions at static and after scanning

Fig. 2. (a) Optical diagram of light-sheet fluorescence microscope; λ/2: half-wave plate; PBS: polarization beam splitter; Slit: slit; L: lens; Pinhole: pinhole filter; SLM: spatial light modulator; GM: galvo mirror; MO: microscope objective; TL: tube lens; F: filters; (b) Experimental setup of light-sheet fluorescence microscope

Fig. 3. Numercial calculation results of Airy light-sheet and planar-Airy light-sheet under 2-PE. (a), (b) The x-z cross-section profile of Airy light-sheet and planar-Airy light-sheet; (c), (d) MTF distribution along the x-axis for Airy light-sheet and planar-Airy light-sheet; (e), (f) Simulation imaging results; (g), (h) Result after deconvolution processing; (i), (l) MTF distribution of Airy light-sheet and planar-Airy light-sheet at different propagation positions (x = 0, 200, 400, 500 μm)

Fig. 4. Imaging results of Gaussian and planar-Airy light-sheet microscope under 2-PE. (a) Imaging results of ordinary light-sheet microscopy under 2-PE; (b) Imaging results of planar-Airy light-sheet microscopy under 2-PE; (c) Graphical representation of the intensity distribution, corresponding to the white dotted line of figure (b); (d) Graphical representation of the intensity distribution, corresponding to the yellow dotted line of figure (b)

Fig. 5. (a) Two-channel single-shot 3D imaging results of zebrafish intracerebral vessels; (b) Images of local cerebrovascular growth during the 44.5-46.5 hpf

Fig. 6. Analysis of the changes of fluorescence intensity of hemorrhagic intracerebral blood vessels and red blood cells with time. (a) Schematic diagram of the changes in the fluorescence intensity of blood vessels and red blood cells in the yellow box (47.5-54 hpf); (b) Fluorescence intensity ratio curves of blood vessels and red blood cells at different time points