1280×1024 EMCCD is a solid-state low light level imaging device with interline transfer structure. It has the ability of all-time imaging and detection from starlight to sunlight environment. It can be widely used in space remote sensing, visual perception and manless driving. However, due to the limitation of device saturation output, when the incident light intensity is too high for EMCCD, blooming takes place, resulting in the reduction of image resolution and affecting the ability of EMCCD to obtain target information. In order to improve the environmental adaptability of EMCCD and avoid the occurrence of blooming, the causes of EMCCD blooming was analyzed, the working principle of vertical antiblooming was introduced, EMCCD pixels with vertical antiblooming structure were designed through theoretical derivation, analysis and numerical simulation, and a 1280×1024 EMCCD device and principle demonstration prototype with vertical antiblooming function were produced. The simulation data and test results show that the vertical antiblooming EMCCD designed in this paper has 500 times antiblooming ability.