Acta Optica Sinica, Volume. 39, Issue 8, 0811001(2019)

Quantitative Polarization Imaging of Zebrafish Muscle Structures

Yu Zhu1, Guang Yang2, Simin Li2, Linbo Wang2, Xin Jin2, Yong Liang2, Weichu Zhao2, and Hui Li2、*
Author Affiliations
  • 1 Department of Precision Machinery and Precision Instrumentation, University of Science and Technology of China, Hefei, Anhui 230026, China
  • 2 Jiangsu Key Laboratory of Medical Optics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, Suzhou, Jiangsu 215163, China
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    Figures & Tables(5)
    Schematic of quantitative polarization imaging setup
    Quantitative polarization imaging method. (a) Schematic of the polarization orientation and light propagation direction; (b) calculation results of detected light intensity dependent on the rotation angle of the orthogonal polarizer/analyzer
    Quantitative polarization imaging of zebrafish muscle tissue. (a) Bright-field image of wild-type zebrafish at 3 days post-fertilization (lateral view); (b) polarization images at a serial rotating angles for the orthogonal polarizer/analyzer; (c) optical intensity at position 1 and position 2 from the serial polarization images, and the corresponding fitting curves; (d) image of quantitative phase retardance
    Comparison of images between wild-type and muscle-damaged zebrafish somite structures. (a) Bright field image of wild type zebrafish at 3 days post-fertilization; (b) phase retardance image corresponding to (a); (c) bright field image of muscle-damaged zebrafish at 3 days post-fertilization;(d) phase retardance image corresponding to (c); (e) average retardance of each somite along the zebrafish abdomen and back, domain represents the number of somite; (f) phase retardance distribution of every
    Muscle growth of wild-type zebrafish and muscle-damaged zebrafish from day 3 to day 7. (a) Phase retardance curves of the 10th somite dependent on time; (b) phase retardance image of wild-type zebrafish dependent on time; (c) phase retardance image of muscle-damaged zebrafish dependent on time
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    Yu Zhu, Guang Yang, Simin Li, Linbo Wang, Xin Jin, Yong Liang, Weichu Zhao, Hui Li. Quantitative Polarization Imaging of Zebrafish Muscle Structures[J]. Acta Optica Sinica, 2019, 39(8): 0811001

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    Paper Information

    Category: Imaging Systems

    Received: Feb. 13, 2019

    Accepted: May. 5, 2019

    Published Online: Aug. 7, 2019

    The Author Email: Hui Li (hui.li@sibet.ac.cn)

    DOI:10.3788/AOS201939.0811001

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