Developing a contact probe for rodent fundus imaging in a confocal scanning laser ophthalmoscope

Xiaoyun Jiang; Yichen Ding; Wenyao Wang; Zhiyu Huang; Zhiru Wang; Elie de Lestrange Anginieur; Yue Yu; Jun Li; Mingliang Pu; Qiushi Ren; Changhui Li

doi:10.3788/COL201614.031701

Chinese Optics Letters, Volume. 14, Issue 3, 031701(2016)

Developing a contact probe for rodent fundus imaging in a confocal scanning laser ophthalmoscope

Xiaoyun Jiang¹, Yichen Ding¹, Wenyao Wang², Zhiyu Huang¹, Zhiru Wang^3,4, Elie de Lestrange Anginieur¹, Yue Yu¹, Jun Li³, Mingliang Pu², Qiushi Ren¹, and Changhui Li^1、*

Author Affiliations

¹Department of Biomedical Engineering, Peking University, Beijing 100871, China

²Department of Anatomy, School of Basic Medical Sciences, Peking University, Beijing 100191, China

³Laboratory Animal Center, Peking University, Beijing 100871, China

⁴School of Public Health, Jilin University, Changchun 130012, China

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Figures & Tables(6)

Fig. 1. Schematic design of the probe. (a) The structure of the contact probe for rats; (b) the surface of the soft gel can slightly change shape to adapt to different rodent eyes.

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Fig. 2. Making the concave surface with a steel ball.

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Fig. 3. Comparison between the flexible contact probe (a, c) and plano–concave method (b, d) in the spot diagram and PSF.

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Fig. 4. Layout of the CSLO system for rodents, and a photo of the contact probe with the subject. L: lens, DM: dichroic mirror, PBS: polarizing beam splitter, QWP: quarter wave plate.

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Fig. 5. In vivo retinal imaging. Comparison of the reflectance image by (a) the flexible contact probe and (b) the plano–concave lens for a rat; (c) autofluorescence imaging and (d) FA for the same rat; (e) reflectance and (d) fluorescence imaging for amouse. Scale bar: 100 μm.

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Table 1. Ocular Parameters for Human, Rat, and Mouse Eyes[22]
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Table 1. Ocular Parameters for Human, Rat, and Mouse Eyes[22]
Average axial length (mm) Total power (D) Average refractive error (D) NA
Human $\sim 23.5 - 24$ $\sim 60$ $\sim 0$ to $+ 1$ $\sim 0.20$
Rat $\sim 6.1$ $\sim 300$ $+ 5$ to $+ 15$ $\sim 0.43$
Mouse $\sim 3.3$ $\sim 560$ $+ 7$ to $+ 15$ $\sim 0.49$

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Xiaoyun Jiang, Yichen Ding, Wenyao Wang, Zhiyu Huang, Zhiru Wang, Elie de Lestrange Anginieur, Yue Yu, Jun Li, Mingliang Pu, Qiushi Ren, Changhui Li, "Developing a contact probe for rodent fundus imaging in a confocal scanning laser ophthalmoscope," Chin. Opt. Lett. 14, 031701 (2016)

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