This study addresses the limitations of low signal collection efficiency and suboptimal signal-to-noise ratio in zero-mode waveguide （ZMW） devices employed for fluorescence detection. A micro-reflector ZMW device was designed and fabricated to enhance the signal-to-noise ratio in fluorescence measurements. Finite element electromagnetic simulations demonstrated a significant improvement in the spatial distribution and intensity of excitation and emission fields within the device. The ZMW apertures and a three-stage micro-reflector structure， with a height of 6.48 μm and a bottom diameter of 4.24 μm， were fabricated using a thick adhesive process followed by exposure etching， complemented by focused ion beam perforation and additional micro-nano fabrication techniques. Fluorescence performance was evaluated using fluorescent microspheres， and detection efficiency was assessed through fluorescence imaging. Quantitative analysis of grayscale intensity and signal-to-noise ratio revealed that the developed device achieved a fluorescence detection signal-to-noise ratio of 119.6， representing an approximate 4.27-fold enhancement compared to conventional ZMW devices without the micro-reflector. This high signal-to-noise ratio ZMW device demonstrates significant potential for applications in weak fluorescence detection and related fields.