Phase-modulated multi-foci microscopy for rapid 3D imaging

Weiqi Wang; Li Gong; Zhiwei Huang

doi:10.1364/PRJ.522712

Photonics Research, Volume. 12, Issue 7, 1548(2024)

Phase-modulated multi-foci microscopy for rapid 3D imaging

Weiqi Wang, Li Gong, and Zhiwei Huang^*

Optical Bioimaging Laboratory, Department of Biomedical Engineering, College of Design and Engineering, National University of Singapore, Singapore 117576, Singapore

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Supplementary Materials

Visualization 1 | Blood flows at 3 depths using the immobilized imaging interval by PM3, equivalent to the conventional multifocal plane microscopy (see Visualization 1)

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Visualization 2 | Blood flows at 3 depths using different imaging intervals by PM3 (see Visualization 2)

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Visualization 3 | 3D Brownian motion of 6 µm fluorescent beads captured by PM3 (see Visualization 3). Image volume: 208 µm × 208 µm × 200 µm. 15 Hz volume rate.

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Weiqi Wang, Li Gong, Zhiwei Huang, "Phase-modulated multi-foci microscopy for rapid 3D imaging," Photonics Res. 12, 1548 (2024)

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Paper Information

Category: Imaging Systems, Microscopy, and Displays

Received: Feb. 29, 2024

Accepted: May. 6, 2024

Published Online: Jul. 1, 2024

The Author Email: Zhiwei Huang (biehzw@nus.edu.sg)

DOI:10.1364/PRJ.522712

CSTR:32188.14.PRJ.522712

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