Laser & Optoelectronics Progress, Volume. 62, Issue 15, 1511002(2025)
Fast Background Removal for Wide-Field Microscopic Imaging Defocus Signal (Invited)
Fig. 1. Simulation of PSF and convolution schematic diagrams in wide-field fluorescent microscopy. (a) 3D rendering of the PSF; (b) cross-sectional views of the PSF; (c) simulated five rings at different depths; (d) depth-color-coded images of five rings; (e) WF image taken by microscope focused on the ring in the middle layer; (f) 3D stack obtained by scanning the five rings layer by layer; (g) depth-color-coded maximum projection of the 3D stack on x-y plane
Fig. 2. Background-removal flowchart. (a) Computational flowchart; (b) schematic diagram of reconstruction process
Fig. 3. Simulated results of line-shaped samples by proposed algorithm. (a1) Sample ground truth (GT) profiles; (a2) samples convolved with the PSF; (a3) processed results of WF images by proposed algorithm (deconv 1); (a4) WF images added Gaussian noise; (a5) processed results of WF images with Gaussian noise by proposed algorithm (deconv 2); intensity profiles of line pair with space of (b1) 90 nm, (b2) 120 nm, (b3) 150 nm, and (b4) 180 nm
Fig. 4. BPAE cell images and intensity results. (a) Depth-color-coded maximum projection of the 3D stack before and after treated by proposed algorithm; enlarged views of the lower-left region in Fig. 4(a), with a single-frame depth of 1.3 μm (b) after processing and (c) before processing; enlarged views of the upper-right region in Fig. 4(a), with a single-frame depth of 1.3 μm (d) after processing and (e) before processing; (f) intensity results along the selected line regions in the enlarged images
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Xinghao Li, Qianbo Liu, Yizhe Hao, Hao Xu, Wenhao Liu, Weisong Zhao. Fast Background Removal for Wide-Field Microscopic Imaging Defocus Signal (Invited)[J]. Laser & Optoelectronics Progress, 2025, 62(15): 1511002
Category: Imaging Systems
Received: Apr. 22, 2025
Accepted: Jun. 3, 2025
Published Online: Jul. 4, 2025
The Author Email: Wenhao Liu (wenhaoliu@hit.edu.cn), Weisong Zhao (weisongzhao@hit.edu.cn)
CSTR:32186.14.LOP251069