The influence of white level and color saturation of LCD smartphone on visual comfort and visual fatigue is researched in this paper. The measurement is performed by the combination of subjective evaluation and objective evaluation, and the principal component analysis is adopted to reduce the dimension of parameters. The results show that the optimal white level of LCD smartphone in the dark environment is about 50 cd/m² with generally higher visual comfort. As to color saturation, the statistically significant discomfort is induced when saturation adjusting coefficient is below 0.7, which brings a degree of visual and physiological discomfort. In addition, LCD cellphone causes the feeling of tiredness both from eye and body after white level experiment while only visual fatigue is found after saturation experiment.