Infrared Spectroscopy Identification of Artificially Aging Wheat Seeds

Fig. 4. 2D-IR of the artificially aging wheat seeds in the range of 1060--1150 cm^-1. (a) Unaged; (b) aging 2 d; (c) aging 4 d; (d) aging 6 d; (e) aging 8 d

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Fig. 5. 2D-IR of artificially aging wheat seeds in the range of 1150--1800 cm^-1. (a) Unaged; (b) aging 2 d; (c) aging 4 d; (d) aging 6 d; (e) aging 8 d

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Fig. 6. PCA score of the artificially aging wheat seeds

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Fig. 7. Clustering results of artificially aging wheat seeds in the range of 1800--800 cm^-1

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Table 1. FTIR absorption peaks of the wheat seeds unit: cm^-1

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Table 1. FTIR absorption peaks of the wheat seeds unit: cm^-1

Untreated wheat seeds	2 d	4 d	6 d	8 d	Functional group and vibration mode	Main compound
3312	3308	3306	3336	3336	υ(O—H)	--
2930	2929	2929	2929	2929	υ_as(—CH₂)	--
1750	1743	1750	1747	1750	υ(C=O)	ester^[21]
1670	1659	1659	1658	1658	--	amide I band of protein^[17]
1542	1542	1542	1542	1542	--	amideⅡband of protein^[17]
1500--1200					--	protein and polysaccharide^[16]
1456	1456	1456	1456	1456	υ(—CH₂)	--
1369	1370	1370	1370	1370	υ(—CH)	--
1242	1243	1243	1243	1243	--	amide Ⅲ band of protein ^[22]
1200--950					--	polysaccharide
1158	1157	1158	1157	1157	υ(C—O,C—C)	--
1081	1081	1081	1081	1081	υ(C—H)	--
1018	1020	1019	1020	1020	υ(C—O),δ(C—OH)	--
950--750					--	carbohydrate isomers
928	930	930	930	931	υ_as(C—O—C)	starch^[23]
765	764	764	763	763	υ(C—C)	--
574	575	574	575	576	--	starch frame vibration^[23]

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Jie Liu, Gang Liu, Shujie Li, Ziang Deng, Quanhong Ou, Youming Shi. Infrared Spectroscopy Identification of Artificially Aging Wheat Seeds[J]. Laser & Optoelectronics Progress, 2021, 58(8): 0830002

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