Experiment Science and Technology, Volume. 23, Issue 4, 33(2025)
Design of a Comprehensive Experiment on Competitive Inhibition in Succinate Dehydrogenase
Figures & Tables(8)
Table 1. [in Chinese]
View tableView in ArticleTable 1. [in Chinese]
试剂 [E]/[S]=2∶1 [E]/[S]=3∶1 [E]/[S]=4∶1 [E]/[S]=5∶1 [E]/[S]=6∶1 空白 0.20 mol/L琥珀酸 400 400 400 400 400 400 0.10 mol/L磷酸盐缓冲液 1600 1200 800 400 0 2400 0.02%亚甲基蓝 40 40 40 40 40 40 粗酶提取液 800 1200 1600 2000 2400 — 石蜡油 1000 1000 1000 1000 1000 1000
Table 2. [in Chinese]
View tableView in ArticleTable 2. [in Chinese]
改进前反应体系 阳性对照 空白对照 改进后反应体系/μL 阳性对照 阴性对照 空白对照 0.20 mol/L琥珀酸 5 5 0.2 mol/L琥珀酸 400 — 400 蒸馏水 5 20 0.1 mol/L磷酸盐缓冲液 400 800 2400 粗酶提取液 15 — 0.02%亚甲基蓝 40 40 40 0.02%亚甲基蓝 2 2 粗酶提取液 2000 2000 — 石蜡油 不定 不定 石蜡油 1000 1000 1000
Table 3. [in Chinese]
View tableView in ArticleTable 3. [in Chinese]
试剂 阳性 [I]/[S]=1∶10 [I]/[S]=1∶1 [I]/[S]=10∶1 [I]/[S]=1∶1 阴性 空白 0.2 mol/L琥珀酸 400 400 400 — — — 400 0.02 mol/L琥珀酸 — — — 400 400 — — 0.2 mol/L丙二酸 — — 400 400 — — — 0.02 mol/L丙二酸 — 400 — — 400 — — 0.1 mol/L磷酸盐缓冲液 400 — — — — 800 2400 0.02%亚甲基蓝 40 40 40 40 40 40 40 粗酶提取液 2000 2000 2000 2000 2000 2000 — 石蜡油 1000 1000 1000 1000 1000 1000 1000
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Jialei SONG, Lingzhi ZENG, Changjun XU, Changfu YANG, Yiying YANG. Design of a Comprehensive Experiment on Competitive Inhibition in Succinate Dehydrogenase[J]. Experiment Science and Technology, 2025, 23(4): 33
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Received: Mar. 8, 2024
Accepted: --
Published Online: Jul. 30, 2025
The Author Email: Yiying YANG (杨奕樱)
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