A new speckle phase singularities method generated by two-dimensional directional wavelet is proposed to measure micro in-plane displacement. A phase singularities image is generated by polarization analysis of twodimensional directional wavelet, setting the rotation angle be π/2 in order to analyze the image before and after displacement of x and y directions, which has network structure stable distribution. The new two-step displacement measurement method is proposed. Preliminary displacement is obtained in the output two correlation points image by using twice Fourier transform and subtraction. The precision displacement of object to be measured is calculated by half the grid width of the initial displacement and displacement between the closest matching singular point. It is studied that simulation and laser mouse positioning experiment. Results show that correct matching singularities are still more than 90% under different noise conditions and the error between actual value and measured value of mouse displacement and direction is less than 0.5 pixel and 1°. This method has many advantages, such as high rate of singularity correct matching, high accuracy, insensitive to noise and good stability, which can precisely locate the displacement and direction of the laser mouse.