Fig. 1. Hyperspectral imaging system
(a): Hyperspectral imager combined with a microscope;(b): Hyperspectral imager combined with a operating microscope

Fig. 2. Spectral denoising
(a): Noise removal by conventional method;(b): Noise removal by SG filtering method

Fig. 3. Range standardization
(a): Before range standardization; (b): After range standardization

Fig. 4. HSI data of the brain in glioma mice
(a): Tricolor composite image of the brain in glioma mice; (b): Reflectance spectra of non-tumorous brain tissues;(c): Reflectance spectra of gliomas brain tissues; (d): Single band images of the brain in glioma mice

Fig. 5. HSI data of H&E stained pathological sections of the brain in glioma mice
(a): Pathological section image of non-tumorous brain tisses and pathological section image of gliomas;(b): Reflectance spectra between gliomas and non-tumorous brain tissues