Acta Physica Sinica, Volume. 68, Issue 21, 218701-1(2019)

Structured illumination for two-dimensional laser induced fluorescence imaging to eliminate stray light interference

Bo Yan, Li Chen, Shuang Chen*, Meng Li, Yi-Min Yin, and Jiang-Ning Zhou
Figures & Tables(7)
Diagram of laser beam transmission changed by rotating glass sheet.旋转玻璃片改变激光光束位置示意图
LIF imaging setup based on the SLIPI technique (A1, 5 × beam expander; RG, Ronchi grating; AR, automatic rotary table; GS, glass sheet (5.3 mm); LS, light system; A, aperture slot; RB, Rhodamine B solution; PF, fliter).基于结构光照明技术的激光诱导发光图像测量装置(A1, 5×扩束镜; RG, Ronchi光栅; AR, 自动旋转台; GS, 玻璃片(5.3 mm); LS, 片光系统; A, 光阑; RB, 罗丹明B溶液; PF, 滤光片)
Simulation process of the PS-SLIPI method: (a) The modulated amplitude value, A; (b) the intensity without grating modulation, SNG = A(R) + B1(R) + B2(R); (c) the intensity with grating modulation, SC; (d) the relative error of modulated amplitude value, A, calculated by LA-SLIPI method, dA = (A1 – A)/A, fc = 0.005 Hz; (e) the error of modulated amplitude value, A, calculated by PS-SLIPI method, dA = AA – A, n = 3; (f) the error of interference components calculated by PS-SLIPI method, dB = BB – B, n = 3.PS-SLIPI方法仿真计算全过程 (a) 调制振幅项A的值; (b) 无光栅调制时的强度值SNG = A(R) + B1(R) + B2(R); (c) 光栅调制后的强度值SC; (d) LA-SLIPI计算后的振幅相对误差值dA/A = (A1 – A)/A, 截止频率fc = 0.005 Hz; (e) PS-SLIPI计算后的振幅误差值dA/A = AA – A, n = 3; (f) PS-SLIPI计算后的干扰分量误差值dB = BB – B, n = 3
Grating fringe changing images based on phase shifting SLIPI method.PS-SLIPI方法的光栅条纹移动图像
(a) Conventional (raw data) LIF images without grating modulation in three different measurement cases; (b) LIF images with grating modulation; (c) images of modulated amplitude value, A, calculated by phase shifting SLIPI method.(a) 三种不同测试环境下的原始LIF图像; (b) 光栅调制LIF图像; (c) PS-SLIPI方法处理后的调制振幅(A)分布图像
(a) LIF images with grating modulation; (b) images of modulated amplitude value, A, calculated by LA-SLIPI method.(a) 不同测量时刻下的光栅调制LIF瞬态图像; (b) LA-SLIPI方法处理后的调制振幅(A)分布的瞬态图像
  • Table 1. Experimental parameters of two SLIPI technique.

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    Table 1. Experimental parameters of two SLIPI technique.

    背景分类激光输出能量相机曝光时间相机增益罗丹明B溶液浓度图像正弦调制周期/像素
    相位移动SLIPI技术Case 1100 mW0.025 s30稳态溶液:1 × 107mol/L 0.058593
    Case 2100 mW0.025 s30稳态溶液:1 × 107mol/L 0.058593
    Case 3100 mW0.025 s30稳态溶液:1 × 107mol/L 0.058593
    基于锁相放大原理的SLIPI技术Case 4100 mW0.025 s30非稳态扩散溶液: 将1 × 106 mol/L溶液注入到1 × 107 mol/L溶液. 0.144536
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Bo Yan, Li Chen, Shuang Chen, Meng Li, Yi-Min Yin, Jiang-Ning Zhou. Structured illumination for two-dimensional laser induced fluorescence imaging to eliminate stray light interference[J]. Acta Physica Sinica, 2019, 68(21): 218701-1

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Paper Information

Received: Jun. 24, 2019

Accepted: --

Published Online: Sep. 17, 2020

The Author Email:

DOI:10.7498/aps.68.20190977

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