Laser & Optoelectronics Progress, Volume. 57, Issue 24, (2020)
Review and Prospect for Single Molecule Localization Microscopy
Figures & Tables(8)
![Schematic of three-dimensional STORM[20]. (a) Experimental setup; (b) relationship between axial lengths of elliptical PSF and z position of molecules](/Images/icon/loading.gif){kind=icon}
Fig. 4. Schematic of three-dimensional STORM[20]. (a) Experimental setup; (b) relationship between axial lengths of elliptical PSF and z position of molecules
Fig. 5. Principle diagram of stochastic photobleaching super-resolution imaging [10]. (a) Attenuation of integrated intensity with time; (b) distinguishing two PSFs by photobleaching
Fig. 6. Setup and principle diagram of MINFLUX[29]. (a) Experimental setup of MINFLUX; (b) detection steps of MINFLUX
Fig. 7. Principle diagram of SIMFLUX[34]. (a) Experimental setup; (b) work flow chart
Table 1. Comparison among all SMLMs
View tableTable 1. Comparison among all SMLMs
SMLM Best resolution Localization precision Advantage Disadvantage PALM [ 16 ]2-25nm σ/ Super high precision Being time-consuming, large amount of calculation, being unsuitable for dynamic process imaging, severe photobleaching, and only exogenous proteins being observed STORM[ 17 ]About 20nm σ/ Super high precision Being limited in in-vivo imaging, severe photobleaching, and being unable to quantify molecule numbers in a cell PAINT[ 18 ]About 25nm σ/ No need to label sample Large amount of calculation, long post-processing time, and only obtaining surface information of sample, not internal information GSDIM[ 22 ]About 30nm σ/ No need of special fluorophore, simple device, and shortening time of image acquisition Severe photobleaching, complex processing, and being limited in real-time imaging of living cells SHRImP [ 10 ]About 10nm σ/ No special fluorophore, and relatively simple process Being unsuitable for cases where two or more neighboring molecules are bleached at the same time and being limited in 2D situation SRRF[ 28 ]50-106nm σ/ Being able to be complemented in conventional microscope Being limited resolution SOFI[ 25 ]100nm σ/ No phototoxicity and short processing time Being limited resolution MINFLUX[ 29 ]1-5nm About L/2 Improved photon utilization, improved localization precision, and being suitable for living cell imaging Complex processing and being limited in sparse solution and long acquisition time SIMFLUX[ 34 ]5-10nm σ/2.4 Improved localization precision and accuracy Being limited in 2D situation ROSE[ 33 ]2-5nm About 50/ Improved localization precision and accuracy Being time-consuming
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Yuzhu Li, Chuankang Li, Xiang Hao, Xu Liu, Cuifang Kuang. Review and Prospect for Single Molecule Localization Microscopy[J]. Laser & Optoelectronics Progress, 2020, 57(24):
Paper Information
Category: Reviews
Received: Apr. 7, 2020
Accepted: May. 8, 2020
Published Online: Dec. 30, 2020
The Author Email: Cuifang Kuang (cfkuang@zju.edu.cn)
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